VARIOUS malformations in rats and mice have been observed to be caused by administration of the vital dye, Trypan blue (Gillman, Gilbert, Gillman, & Spence, 1948; Fox & Goss, 1956, 1957; Hamburgh, 1952, 1954; Waddington & Carter, 1952, 1953). Recently Waddington & Perry (1956) reported a teratogenic effect of Trypan blue on Amphibian embryos. The present paper deals with the effects of this dye...

We present a robust method to fuse in parallel an array of anchored droplets in a microchannel. Pairs of anchored droplets are fused by the removal of surfactant from the droplet interface by reducing the surfactant content in the flowing external oil phase. By controlling the flow of multiple oil inlets, the selective fusion of rows of droplets in a larger array is demonstrated. The technique ...

compounds containing triazene ring structure are cytotoxic agents and clinically used as antitumor alkylating agents. in this study, a series of triazene derivatives holding alkyl and aryl moieties were synthesized and proved to be potent cytotoxic agents in-vitro particularly against eight cancer cell lines (pc3, ht29, hela, hl60, jurkat, k562, mcf7, hepg2) and a non-cancerous cell line (huvec...

Trypan Blue is one of the most commonly used dyes for assessing cell viability by dye exclusion assays. Dye exclusion assays are based on the principle that an intact cell membrane is necessary for the exclusion of certain dyes. Some other dyes used for this purpose include safranin, eosin, Congo Red, erythrocin, nigrosin and Alcian Blue (5). Pappenheimer (4) was the first to describe the use o...