About 60–70% of the organic matter contained by domestic wastewater is formed by lipids and proteins. The microorganisms that are able to degrade this organic matter produce hydrolytic enzymes that cannot be produced by standard cultivation techniques. The extraction methods have to include detergents or ionic exchange resins to recover qualitatively and quantitatively the enzyme activity. In t...

Membrane-associated phosphatidylserine synthase (CDP-diacylglycero1:L-serine O-phosphatidyltransferase, EC 2.7.8.8) was purified from the microsomal fraction of Saccharomyces cerevisiae strains S288C and VALBC(YEpCHO1). VALBC(YEpCHO1) contains a hybrid plasmid bearing the structural gene for phosphatidylserine synthase and overproduces the enzyme 6-7-fold (Letts, V. A., Klig, L. S., Bae-Lee, M....

The aim of this study was to evaluate the impact of different inactivation and splitting procedures on influenza vaccine product composition, stability and recovery to support transfer of process technology. Four split and two whole inactivated virus (WIV) influenza vaccine bulks were produced and compared with respect to release criteria, stability of the bulk and haemagglutinin recovery. One ...

Background: Xanthomonas campestris is a biopolymer producing gram negative bacterium. Production of xanthan biopolymer can be affected by different extrinsic factors as well as surfactants. Hitherto, effects of nonionic surfactants on xanthan production have been studied in a limited number of articles.Objective: In the present study, nonionic surfactants...

Aiming the production of lipopeptides biosurfactants (BioS), we isolated a new strain identified as Bacillus subtilis ZNI5 from hydrocarbon contaminated soil. Lipopeptides purification by anionic exchange chromatography and identification Reverse Phase High Performance Liquid Chromatography–Mass Spectrometry (RP-HPLC–MS) permitted selection different homologues divided into four families. The f...

OBJECTIVES/HYPOTHESIS To verify the reliability and validity of automated scoring and compare it to that of manual scoring for diagnosing obstructive sleep apnea using an Embletta X100 level 2 portable device. STUDY DESIGN Retrospective study. METHODS A total of 116 patients with suspected obstructive sleep apnea who had successfully received portable polysomnography with the Embletta X100 ...

Iron, cobalt and copper are metals, which appear together in many real samples, both natural and artificial. Recently a classical univariate micellar colorimetric method has been developed for determination of these metal ions. The organized molecular assemblies such as micelles are used in spectroscopic measurements due to their possible effects on the systems of interest. The ability of mi...

iron, cobalt and copper are metals, which appear together in many real samples, both natural and artificial. recently a classical univariate micellar colorimetric method has been developed for determination of these metal ions. the organized molecular assemblies such as micelles are used in spectroscopic measurements due to their possible effects on the systems of interest. the ability of micel...