We previously demonstrated that the gap junction protein connexin43 is translated as a 42-kD protein (connexin43-NP) that is efficiently phosphorylated to a 46,000-Mr species (connexin43-P2) in gap junctional communication-competent, but not in communication-deficient, cells. In this study, we used a combination of metabolic radiolabeling and immunoprecipitation to investigate the assembly of c...

Several species of Gram-positive bacteria have cell wall peptidoglycan (syn. murein) in which not all of the sugar moieties are N-acetylated. This has recently been shown to be a secondary effect, caused by the action of a peptidoglycan N-acetylglucosamine deacetylase. We have found that the opportunistic pathogen Listeria monocytogenes is unusual in having three enzymes with such activity, two...

We describe a new branch predictor that is designed to balance multiple constraints—predicting branch biases versus predicting specific branch instance behavior. Most branch instances only require branch bias information for accurate predictions while a select few require more sophisticated prediction structures. Our predictor uses a cache mechanism to classify branches and dynamically adjust t...

Succinate dehydrogenase (SDH) was solubilized from membranes of Mycobacterium phlei by Triton X-100 with a recovery of about 90%. The solubilized SDH was purified about 90-fold by Sephacryl S-300, DEAE-cellulose, hydroxylapatite, and isoelectric focusing in the presence of Triton X-100 with a 20% recovery. SDH was homogeneous, as determined by polyacrylamide gel electrophoresis in nondenaturing...

Triton X-100 is widely but not universally used in NADPH-diaphorase histochemical staining. We investigated its effect on the staining and examined nitroblue diformazan (NBF) production under the influence of Triton X-100. Exposure of opossum esophagus, intestine, and colon tissues to Triton X-100 before staining enhanced staining of nerve cells and fibers and suppressed staining of non-neural ...

Mycobacterium tuberculosis grew in the presence of toxic concentrations of free fatty acids when an adequate amount of triton WR1339 was added to the medium. Triton produced a solubilizing effect on turbid suspensions of fatty acids, indicating the formation of a detergent-lipid complex. Oleic acid complexed with triton served as a source of carbon for growth of M. tuberculosis.

When ‘25l-labeled blood platelets were lysed with an equal volume of 2% Triton X-100, 10 mM EGTA, and 100 mM Tris, pH 7.4. the Triton-insoluble residue (“cytoskeleton”) contained about 1 1 % of the platelet protein. predominantly actin and actin-binding protein, 3.5% of the platelet radioactivity, and less than 2% of the platelet lipid phosphorus. Radioautographs of polyacrylamide gels of this ...

Context. Jeans escape is believed to dominate atmospheric for most outer Solar System bodies. However, non-thermal mechanisms, particularly and ionospheric chemistry, are likely contribute substantially neutral on Triton. Aims. This study devoted evaluating the role of chemically induced H, H 2 , C, N, O, N CO Triton via a variety processes. Here, we also aim identify dominant processes these s...