A potential biomarker for early diagnosis of cancer is assessment of high nuclear DNA content. Conventional hematoxylin staining is neither stoichiometric nor reproducible. Although feulgen stain is stoichiometric, it is time consuming and destroys nuclear morphology. We used acidic thionin stain, which can be stoichiometric and also preserve the nuclear morphology used in conventional cytology...

Ž . Diploid and triploid all-female Atlantic salmon Salmo salar smolts were challenged with 2.5 h of confinement stress in aerated seawater. Subsequent stress responses were quantified by Ž . plasma cortisol, glucose, and lactate, and by the haematological parameters haematocrit Hct , red Ž . Ž . Ž . blood cell count RBCC , mean cell volume MCV , blood haemoglobin concentration Hb , mean Ž . Ž ...

We report the occurrence of triploid preimplantation embryos following in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) in a woman with two previously-identified triploid conceptuses which spontaneously underwent fetal demise at 10 and 23 weeks' gestation. An error in maternal meiosis II is proposed as the most likely cause.

Because of sexual propagation of Mexican lime in southern regions of Iran, there are many lime accessions (lime biotypes) in Mexican lime gardens in which appear some variation in fruit and tree shape. However, most of these accessions are susceptible to witches broom disease of lime (WBDL). Persian lime (C. latifolia Tanaka) is a triploid WBDL-tolerant species. Considerable number of un-descri...

We used flowcytometry to ploidy verification after that investigate difference between diploid and spontaneous triploid through the truss dimension and classical dimension at crucian carp, Carassius auratus, crucian carp C. cuvieri and common carp, Cyprinus carpio collected from Hangang river, Hantangang river, Imjingang river, Geumgang river, Yeongsangang river and Nakdonggang river, Korea. Th...

Between 1 and 4% of human oocytes fertilized in vitro are tripronuclear. It has been reported that these tripronuclear oocytes can develop to grossly normal-appearing morulae and that chromosomally, these embryos could be triploid, diploid, or severely depleted. The etiology and proportion of apparently diploid and aneuploid embryos deriving from tripronuclear human oocytes is unknown. This stu...

The scarcity of parthenogenetic vertebrates is often attributed to their 'inferior' mode of clonal reproduction, which restricts them to self-reproduce their own genotype lineage and leaves little evolutionary potential with regard to speciation and evolution of sexual reproduction. Here, we show that for some taxa, such uniformity does not hold. Using hybridogenetic water frogs (Pelophylax esc...

HE sterility of triploids and other unbalanced polyploids is generally conT ceded to be a consequence of irregularities in the distribution of univalents and multivalents at meiosis. Occasional viable gametes are produced, however, because of nonrandom orientation and segregation of chromosome associations. Thus, SATINA and BLAKESLEE (1937a, b) found a higher frequency of n and 2n chromosome nu...