We use a combination of calorimetric and volumetric techniques to detect and to characterize the thermodynamic changes that accompany helix-to-coil transitions for five polymeric nucleic acid duplexes. Our calorimetric measurements reveal that melting of the duplexes is accompanied by positive changes in heat capacity (DeltaCP) of similar magnitude, with an average DeltaCP value of 64.6 +/- 21....

Trehalose is a non-reducing sugar that plays an important role in plant growth and development. To study the role of trehalose on lipid metabolism and gluconeogenesis, Arabidopsis thaliana wild type (WT) and TreF (a line expressing trehalase) were grown on ½ MS medium with or without 100 mM sucrose and or trehalose in light or continuous darkness. In dark, trehalose leads skotomorphoge...

Thompson, L.A. Strasheim and R.R. Burgess. 1990. Purification and characterization of proximal sequence element-binding protein 1, a transcription activating protein related to Ku and TREF that binds the proximal sequence element of the human U1 promoter. J. Biol. Chem. 265:17911-17920. 20.May, G., C. Sutton and H. Gould. 1991. Purification and characterization of Ku-2, an octamer-binding prote...

On October 15, 1998, Marquette University Law School convened a symposium for consideration of the findings of the MacArthur Foundation Research Network on Mental Health and the Law, which has members in numerous locations throughout the United States. The symposium was attended by an interdisciplinary audience that included psychologists, psychiatrists, lawyers, judges, social workers, and men...

The dynamic optimization of microwave heating of foods is considered. Two classes of problems (maximum temperature uniformity and maximum quality uniformity) are stated here. Results obtained using a deterministic method, an stochastic method and new hybrid method are reported. It is shown that, for the models and food loads considered, it is theoretically possible to achieve a quite uniform fi...

Myoglobin (Mb) is a heme protein that carries small-molecule ligands such as O2, CO and NO in muscles, and can be considered as a subunit of hemoglobin, a paradigm protein for the study of allostery. Due to its small size, availability and photosensitivity of the heme–ligand bond, Mb has served as a prototypical model system for studying protein structural dynamics. Accordingly, structural dyna...