One concern about using transgenic plants is the genetic variation that occurred from theirs tissue culture and regeneration. Molecular markers are an important element for efficient and effective determination of genetic variation. The present work was carried out to assess the genetic uniformity of transgenic cottons (Bt and chitinase lines), using RAPD, ISSR molecular markers and SDS-PAGE an...

Abstract Background: Selectable marker gene (SMG) systems are critical for generation of transgenic crops. Transgenic crop production Background: Selectable marker gene (SMG) systems are critical for generation of transgenic crops. Transgenic crop production without using SMG is not economically feasible. However, SMGs are non-essential once an intact transgenic plant has been established. Eli...

in this study, an efficient agrobacterium-mediated transformation method was developed forpomegranate (punica granatum l.), a difficult-to-transform plant. in vitro shoot segments wereinoculated with agrobacterium tumefaciens strain lba4404 harboring the binary vectorpbi121 carrying the neomycin phosphotransferase (nptii) gene as a selectable marker and β-glucuronidase (gus) gene as a reporter....

one concern about using transgenic plants is the genetic variation that occurred from theirs tissue culture and regeneration. molecular markers are an important element for efficient and effective determination of genetic variation. the present work was carried out to assess the genetic uniformity of transgenic cottons (bt and chitinase lines), using rapd, issr molecular markers and sds-page an...

environmental stresses trigger a wide variety of plant responses, such as altered gene expression to changes in cellular metabolism and growth. the several plant reactions exist to circumvent the potentially harmful effects caused by light, drought, salinity, pathogen infections and other stresses. a-tocopherol is the major vitamin e compound found in leaf chloroplasts. this antioxidant deactiv...

polychlorinated biphenyls (pcbs) are chlorinated biphenyl rings that due to their properties such as heat resistance and stability were widely used in different industries from 1920s to 1980s. due to their characteristics such as stability, resistance to decomposition and harmful effects on human health, their production was banned in 1980s., worldwide contamination of water and soil with pcbs ...

plant tissue culture techniques are used as basic requirement of common plant transformation systems. in most cases of plant transformation, a reproducible regeneration protocol is the limiting step due to long time lasting, specialized facilities and well experienced persons. furthermore, tissue culture procedures induce somaclonal variation among regenerated transgenic plants. therefore, rece...

expansins are cell wall proteins that due to changes in ph, causing the expansion of the cell wall .these proteins are causing deformation of walls through loosening hydrogen bonds between the cellulose and polymer matrix microfibrils .in this study, a root specific gene called atexpa18 -that directly affect the formation of root hairs and also its role  have been proved in other parts of the p...