The kinetics of the hydrolysis of Woodward’s Reagent K has been studied as a function of pH in aqueous solution. Rate constants for the reagent transformation have been combined with rate data for loss of staphylococcal nuclease activity to provide the mechanism for the protein modification. The reactive species for derivatization of the enzyme is the intermediate ketoketenimine. The pH depende...

this study was aimed to evaluate the efficacy of dipstick tests (leukocyte esterase and nitrite) in diagnosis of spontaneous bacterial peritonitis (sbp) in cirrhotic patients. forty six children with ascites hospitalized between 2009 and 2010 in children medical center were enrolled in this study. reagent strip assays for leukocyte esterase and nitrite were performed on ascetic fluid and the re...

Several homogeneous photo-based advanced oxidation processes-namely photolysis, photo-oxidation, and photo-Fenton oxidation—were investigated for the elimination of ibuprofen in water. The effects several operating parameters, such as lamp type (low or medium pressure mercury, xenon-arc), concentration hydrogen peroxide (0.5 to 7 times stoichiometric amount required mineralization), Fenton reag...

Software automated genomic engineering (SAGE) enables arbitrary genetic modification of cells on a fluidic platform that implements the multiplex automated genomic engineering (MAGE) process [1]. Electrowetting-ondielectric (EWD) digital microfluidics is well suited for SAGE because of its inherent reconfigurability, small reagent volumes, and parallel processing capability [2]. We report on th...