نتایج جستجو برای: total protein concentration elisa
تعداد نتایج: 2254454 فیلتر نتایج به سال:
A sensitive sandwich-type enzyme-linked immunosorbent assay (ELISA) for human plasma phospholipid transfer protein (PLTP) has been developed using a monoclonal capture antibody and a polyclonal detection antibody. The ELISA allows for the accurate quantification of PLTP in the range of 25-250 ng PLTP/assay. Using the ELISA, the mean plasma PLTP concentration in a Finnish population sample (n = ...
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OBJECTIVE To compare serum total thyroxine (T4) concentrations obtained with an in-house ELISA and a validated radioimmunoassay (RIA). DESIGN Laboratory trial. SAMPLE POPULATION 50 canine and 50 feline serum samples submitted for measurement of total T4 concentration with the RIA; samples were selected to represent a wide range of concentrations (< 6 to 167 nmol/L). PROCEDURE Results of t...
The aim of this study was evaluated the effect selected bioactive substances and nanoparticles on immunoreactivity edible packages based chitosan using ELISA. analysed protein tropomyosin. results confirmed presence tropomyosin (3.77 ± 0.79–5.75 0.01 µg/g) in control samples. This demonstrated that form grape (0.61 0.34–0.43 0.16 µg/g), blueberry (0.58 0.32–0.39 0.27 parsley extracts (2.09 1.28...
BACKGROUND Alzheimer's disease (AD) is the most common cause of dementia in Western countries and in Japan. Numerous blood and cerebrospinal fluid (CSF) tests based on the disease pathology have been proposed for early detection of AD. By comparing the CSF proteome of AD patients and controls it might be possible to identify proteins that play a role in the disease process and thus study the pa...
background: as a drug target and an antigenic agent, hiv-1 protease (hiv-1 pr) is at the center of attention for designing anti-aids inhibitors and diagnostic tests. in previous studies, the production of the recombinant protease has been faced with several difficulties; therefore, the aims of this study were the easy production, purification of the soluble form of protease in e. coli and inves...
the aim of this study was to compare the total protein levels in serum of veteran exposure to sulfur mustard (sm) which was used in the iran-iraq war. in this study, 35 patients exposed to mustard gas (case group) and 35 healthy subjects (control group) were evaluated. the total protein concentration was measured by bradford method. the average amount of protein in serum exposure to sulfur must...
chaperones are produced by prokaryotic, yeast and higher eukaryotic cells for various purposes. over-expression of each chaperone or sets of them affect the production level of a recombinant protein in the cell. on the basis of this hypothesis, five different plasmids with 5 different combinations of 6 chaperones molecule, transformed into escherichia coli along with human basic fibroblast grow...
A previous nationwide Chinese epidemiological study revealed through isolation of A‑B+ Clostridium difficile strains, which produce toxin B (TcdB), but not toxin A TcdA, that the strains are widespread and more frequent in east Asian countries,. The development of a process capable of detecting TcdB is required in microbiological laboratories in order to facilitate the control of the A‑B+ C. di...
Recently we have shown the antiproliferative activity of Gleditsia sinensis fruit extract (GSE) on various solid tumour and leukaemia cell lines as well as primary cultured bone marrow cells isolated from patients with acute and chronic myelogenous leukaemia. We further studied whether the growth inhibitory effect of GSE involves basic fibroblast growth factor (bFGF) in cancer cell lines includ...
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