AND SUMMARY Low incidence and levels of aflatoxin were identified in corn of all grades grown in the Midwest in 1964, 1965, and 1967. Later surveys indicate that corn grown in southern regions is subject to invasion by Aspergillus flavus and subsequent aflatoxin formation. This mycotoxin is formed either in the field or in storage. In the field, such factors as insect damage and weather conditi...

Aflatoxins, carcinogenic toxins produced by Aspergillus fungi, contaminate maize, peanuts, and tree nuts in many regions of the world. Pistachios are the main source of human dietary aflatoxins from tree nuts worldwide. Over 120 countries have regulations for maximum allowable aflatoxin levels in food commodities. We developed social network models to analyze the association between nations' af...

the aim of this study was to evaluate the utility of a proficiency testing program among limited number of local laboratories as an alternative to the iupac/citac guide on proficiency testing with a limited number of participants, specially where international schemes are not accessible. as a sample scheme we planned to determine aflatoxins (b1, g1, b2, g2, total) in iranian pistachio matrix. a...

Peanuts (Arachis hypogea L.) are important substrates for the growth and subsequent aflatoxin production by different members of Aspergillus. The aims of the current study were to identify the toxigenic fungi associated with peanut and to study the effect of ozone gaseous (O3) on fungal spoilage and aflatoxin concentration in peanuts. Peanut samples were collected from three Egyptian governorat...

Aspergillus flavus ATCC 15517 produced up to 212 mg per liter of total aflatoxin in submerged culture in aerated (3,000, 6,000, 9,000, and 12,000 ml/min) and agitated medium in 14-liter fermentors with 10 liters of medium consisting of 2% yeast extract and 10% sucrose. Aflatoxin production increased with time. A maximum of 212 mg/liter was produced at 9,000 ml/min aeration, whereas the yield de...

Antibody based techniques are widely used for the detection of aflatoxins which are potent toxins with a high rate of occurrence in many crops. We developed a murine monoclonal antibody of immunoglobulin A (IgA) isotype with a strong binding affinity to aflatoxin B1 (AFB1), aflatoxin B2 (AFB2), aflatoxin G1 (AFG1), aflatoxin G2 (AFG2) and aflatoxin M1 (AFM1). The antibody was effectively used i...

Different in vitro and in silico approaches from our research group have demonstrated that neutral electrolyzed water (NEW) can be used to detoxify aflatoxins. The objective of this investigation was to evaluate the ability of NEW to detoxify B-aflatoxins (AFB₁ and AFB₂) in contaminated maize and to confirm detoxification in an in vivo experimental model. Batches of aflatoxin-contaminated maize...

The molecular regulation of aflatoxin biosynthesis is complex and influenced by several environmental conditions; one of these is temperature. Aflatoxins are produced optimally at 28-30 C, and production decreases as temperatures approach 37 C, the optimum temperature for fungal growth. To better characterize the influence of temperature on aflatoxin biosynthesis, we monitored the accumulation ...

A total of 260 isolates, including 43 species of Penicillium and 7 species of Aspergillus, were screened for their ability to produce aflatoxin on rice. Chloroform extracts were analyzed by thin-layer chromatography. None of the isolates produced aflatoxin. Certain species of Penicillium produced fluorescent substances that either were similar in R(F) or were of similar color to B and G aflatox...