In this work, optical retardation (OR) was investigated in corneal stroma collagen fibers from nonobese diabetic (NOD) and healthy (BALB/c) mice. Linear dichroism (LD) was investigated in corneas stained with Ponceau SS and Toluidine Blue, using microspectrophotometer. NOD corneas showed higher OR values than the control. Ponceau SS-complexed collagen fibers showed positive LD that was higher f...

Difficulties in obtaining reproducible and accurate enumeration of circulating basophils with existing techniques have hampered investigation of this infrequent cell population. A new basophil staining method is described that employs alcian blue dye for staining of heparin within basophils at low pH and in the presence of lanthanum ions. Basophil recognition is facilitated by reducing nonspeci...

A bleeding disorder occurring during labour and affecting mother and foetus is described. All stages of coagulation were normal until the reaction fibrinogen-->fibrin. Either there was some abnormality present in the fibrinogen molecule, or there was an "anticoagulant" acting at this stage. The abnormality was reversible by protamine sulphate and toluidine blue, but in other respects did not re...

The photodynamic action of dyes upon biological systems has been reviewed by Blum (1941). Inactivation of bacteriophage by this process has been studied by Clifton (1931), Perdrau and Todd (1933), Burnet (1933), Morales (1941), and Pacheco and Morales (1941). More recently, Welsh and Adams (1954) and Yamamoto (1956, 1958) investigated the kinetics of photodynamic inactivation of coliphage. In t...

Gold nanorods conjugated with a hydrophilic photosensitizer, toluidine blue O, acted as dual-function agents in photodynamic inactivation and hyperthermia against methicillin-resistant Staphylococcus aureus.

Background: Mast cells are involved in multiple biological events. The significance of mast cells in non-neoplastic and neoplastic lesions of the cervix has been studied with conflicting results. Its presence in a tumor has been described as evidence of host immunologic antitumor response, angiogenesis and tumor invasion. Aims/Objectives: To study mast cell density in various cervical lesions u...

A one-pot synthesis of 2,6-dibromodithieno[3,2-b;2',3'-d]thiophene (dibromo-DTT, 4) was developed. A key step was bromodecarboxylation of DTT-2,6-dicarboxylic acid, obtained by saponification of the diester 1. The donor-acceptor dye DAHTDTT (13), based on a central 2,6-bis[2'-(3'-hexylthienyl)]dithieno[3,2-b;2',3'-d]thiophene core (9), was prepared and incorporated in a dye-sensitized solar cel...