In order to conduct a comparative proteomics study of human nasopharyngeal carcinoma (NPC) to understand the molecular mechanisms that participate in the formation of NPC, the two-dimensional gel electrophoresis (2-DE) reference map of human NPC tissue proteome was described. To provide a high level of reproducibility between gels and accurately array each protein expressed in NPC tissue proteo...

were re-crystallized with ethanol/acetone/0.1%TFA (60:30:10, v:v). MS and MS/MS spectra were obtained fully automatically on an ultraflex MALDI–TOF/TOF mass spectrometer (Bruker Daltonics). The WARP–LCTM software controlled the intelligent precursor ion selection and BioTools 3.0 was used for ESI and MALDI spectra validation (both Bruker Daltonics). Database search: Mascot Version 2.0.05 (www. ...

A novel lumazine peptide, penilumamide (1), was isolated from the fermentation broth of a marine-derived fungal strain, identified as Penicillium sp. (strain CNL-338) and the structure of the new metabolite was determined by analysis of ESI-TOF MS data combined with 1D and 2D NMR experiments.

Department of Chemistry, Graduate Schoo Kyoto 606-8502, Japan. E-mail: osuka@kuc Spectroscopy Laboratory for Functional p Chemistry Yonsei University, Seoul 120-749 † Electronic supplementary information methods, HR-ESI-TOF mass spectra, UV cyclic voltammograms, X-ray crystal struc CCDC 1025770, 1025771 and 1025772. Fo or other electronic format see DOI: 10.103 Cite this: Chem. Sci., 2015, 6, 1696

Direct mass spectrometric analysis of complex biological samples is becoming an increasingly useful technique in the field of proteomics. Matrix-assisted laser desorption/ionization mass spectroscopy (MALDI-MS) is a rapid and sensitive analytical tool well suited for obtaining molecular weights of peptides and proteins from complex samples. Here, a fast and simple approach to cellular protein p...

A method is developed toward high sequence coverage of proteins isolated from human breast cancer MCF10 cell lines using a 2-D liquid separations. Monolithic-capillary columns prepared by copolymerizing styrene with divinylbenzene are used to achieve high-resolution separation of peptides from protein digests. This separation is performed with minimal sample preparation directly from the 2-D li...

Matrix-assisted laser desorption/ionization (MALDI) and electrospray ionization (ESI) time-of-flight mass spectrometry (TOFMS) play an essential role in the analysis of biological molecules, not only peptides and proteins, but also DNA and RNA. Tandem mass spectrometry used for sequence analysis has been a major focus of technological developments in mass spectrometry, but accurate mass measure...

An aqueous normal phase HPLC method coupled with UV or ESI/MS detection was used for the determination of a wide variety of nucleotides, essential in metabolomics studies. Fifteen nucleotides were tested in clinically relevant mixtures at levels of 100 microg/mL for UV detection and 1 microg/mL for ESI-MS detection. Analysis times for all protocols developed were less than 20 min. The chromatog...