CRISPR/Cas12a-based detection is a novel approach for the efficient, sequence-specific identification of viruses. Here we adopt use CRISPR/Cas12a to identify tomato brown rugose fruit virus (ToBRFV), new and emerging tobamovirus which causing substantial damage global industry. Specific CRISPR RNAs (crRNAs) were designed detect either ToBRFV or closely related mosaic (ToMV). This technology ena...

Any individual virus can infect only a limited range of hosts, and most plant species are "nonhosts" to a given virus; i.e., all members of the species are insusceptible to the virus. In nonhost plants, the factors that control virus resistance are not genetically tractable, and how the host range of a virus is determined remains poorly understood. Tomato (Solanum lycopersicum) is a nonhost spe...

Virus-like symptoms were recently observed on leaves of landscape and nursery downy and star jasmine (Jasminum multijlorum) and wax jasmine (J. gracile) in southeast Florida. Foliar symptoms included mottling, chlorotic ring spots and chlorotic line patterns. An agent was mechanically transmitted with difficulty from symptomatic leaves of downy jasmine to Nicotiana debneyi and N. tabacum 'Xanth...