Both ornithine decarboxylase inhibition to deplete polyamines and cyclooxygenase inhibition diminish the migration response to injury of human airway epithelial cells in tissue culture monolayers by approximately 75%. Restoration of normal migration responses is achieved in the polyamine depleted system either by exogenous reconstitution of polyamines or the addition of prostaglandin E(2) (PGE(...

Regulatory T cells (Treg) are a critical immune component guarding against excessive inflammatory responses, but current understanding of Treg heterogeneity and function in non-lymphoid tissue humans is limited. In this project we characterising unique types signatures the context tissue-confined responses human skin gut. We hypothesising that common ground between these pathologies may be foun...

Ornithine decarboxylase (ODC) and polyamines are intimately involved in normal cellular proliferation and are likely to play a role in carcinogenesis. ODC activity and polyamine content were measured in tissue samples obtained during colonoscopy from 48 benign neoplastic polyps (20 tubular adenomas; 28 villous adenomas), 18 cancers (including 5 malignant polyps), and adjacent mucosa. ODC activi...

Several new polyamine analogues have been developed for the treatment of human solid tumors. The phenotypespecific activity of some of these analogues has been associated with the superinduction of the rate-limiting enzyme in polyamine catabolism spermidine/spermine N-acetyltransferase (SSAT). Using immunohistochemistry, we found a majority (64%) of human primary lung cancer explants to exhibit...

Spermidine/spermine-N(1)-acetyltransferase (SSAT) regulates cellular polyamine content. Its acetylated products are either excreted from the cell or oxidized by acetylpolyamine oxidase. Since polyamines play critical roles in normal and neoplastic growth and in ion channel regulation, SSAT is a key enzyme in these processes. SSAT is very highly regulated. Its content is adjusted in response to ...

Pegg AE. Spermidine/spermine-N-acetyltransferase: a key metabolic regulator. Am J Physiol Endocrinol Metab 294: E995–E1010, 2008. First published March 18, 2008; doi:10.1152/ajpendo.90217.2008.—Spermidine/spermine-N-acetyltransferase (SSAT) regulates cellular polyamine content. Its acetylated products are either excreted from the cell or oxidized by acetylpolyamine oxidase. Since polyamines pla...

The relationship between polyamine synthesis, growth and secretion in vivo was examined in ventral prostates from: (a) intact rats aged 3-60 weeks; (b) animals castrated for 7 days before injection with 5r-dihydrotestosterone (17,8-hydroxy-5a-androstan-3-one), testosterone and 5 r-androstane-3,8,17,f-diol for up to 10 days; (c) rats injected with the 3fi,17fidiol immediately after castration. O...

background: the aim of this study was to investigate the preventive effect of the polyamine component n, n, n´, n´-tetrakis(2-aminoethyl)2,2-dimethylpropane-1,3-diamine (tdmtn) on apoptotic motor neurons in adult mouse spinal cord slices. materials and methods: thoracic region of adult mouse spinal cords was sliced by a tissue chopper into 400 μm slices and cultured in medium in the presence or...

DFMO is an irreversible inhibitor of ornithine decarboxylase (ODC), the key enzyme in mammalian polyamine biosynthesis. The goal of this study was to determine the effects of DFMO 0.5 g/m2/day as a single oral dose on polyamine and ODC levels in rectal, rectosigmoidal, and cecal colonic mucosae of individuals at risk for colon cancer because of a personal history of adenomatous polyps of the co...