Optimisation of the reagents used to perform PCR is critical for reliable and reproducible results. As with any PCR initial time spent on optimisation of a real-time assay will be benefi cial in the long run. Specifi city, sensitivity, effi ciency and reproducibility are the important criteria to consider when optimising an assay and these can be altered by changes in the primer concentration, ...

Real-time PCR is a powerful tool to compare relative transcriptional abundance, and has been broadly applied in biomedical sciences. However, methods for rigorous statistical analyses have lagged behind applications. Confidence interval and statistical significance considerations are not explicit in many of the current data analysis approaches. Based on the standard curve method and other usefu...

The real-time polymerase chain reaction (real-time PCR) is a recent modification to PCR (UNIT 10.2) that is rapidly changing the nature of how biomedical science research is conducted. It was first introduced in 1992 by Higuchi and coworkers and has seen a rapid increase in its use since (Higuchi et al., 1992, 1993). Real-time PCR allows precise quantification of specific nucleic acids in a com...

background: stem cells from human exfoliated deciduous teeth (shed) have the capability to differentiate into neural cells. neurotrophins including nerve growth factor (ngf), brain-derived neurotrophic factor (bdnf), neurotrophin-3 (nt-3), and neurotrophin-4 (nt-4) have neurogenesis, neurotrophic, or neuroprotective effects and are expressed in developing teeth. the aim of this study was to mea...

asthma is a chronic inflammatory disorder of the airways. the stress is a factor for asthma which indicates a disorder in the function of communicational mediators of nervous and  immunological systems  such  as  neurotransmitters.  a  study  indicated  that  blood serotonin concentration increases in asthmatic patients. other study indicates that one kind of the serotonin  receptors, named 5ht...

background: human bocavirus ( hbov) infection is of worldwide distribution. there is increasing evidence that hbov is pathogenic for the human gastroenteric tract. however, less data are available on the role of hbov in gastroenteritis. the present study was aimed to determine the prevalence of hbov in children with gastroenteritis.   methods : real-time pcr taqman was used to screen 200 stool ...

the plaque forming unit (pfu) and tissue culture infectious dose50 (tcid 50 ) methods are used for evaluation of vaccine heat stability and effect of various stabilizers on thermal stability of vaccines. the aim of present study is using real-time pcrtechniquefor estimation of vaccine degradation rate and thermal stability of measles vaccines. lyophilized measles vaccines containing three vario...

background and aims: prostate cancer is the second most common cancer in men worldwide in men. matrix metalloproteinase 2 (mmp2) has a role in the invasion and destruction of the basement membrane and the extra-cellular matrix and facilitating the process of tumor cell invasion. the present study was conducted to compare the expression of mmp2 gene in prostate cancer (pca) and benign prostatic ...

introduction: the protozoan parasite, cryptosporidium spp., widely spreads in both raw and drinking waters. it is the causative agents of waterborne diarrhea and gastroenteritis in the world. in the present study, a molecular assay was used for the detection and quantification of cryptosporidium oocysts in environmental water samples. materials and methods: thirty surface water samples were col...