Threonine oxidation to glycine was investigated in vivo in twelve growing pigs (27.4 kg live weight) fed on one of the following three diets with graded levels of threonine supply: a low-threonine diet (LT), a control well-balanced diet (C) or a high-threonine diet (HT), during 10 h constant infusion of L-[1-13C]threonine and [2-3H]glycine in the cranial vena cava and [1-14C]glycine in the port...

Threonine dehydratase converts L-threonine to 2-ketobutyrate. Several threonine dehydratases exist in bacteria, but their origins and evolutionary pathway are unknown. Here we analyzed all the available threonine dehydratases in bacteria and proposed an evolutionary pathway leading to the genes encoding three different threonine dehydratases CTD, BTD1 and BTD2. The ancestral threonine dehydrata...

Experiments were conducted to estimate the threonine requirement of pigs weighing 5 to 15 kg and to determine whether excess methionine increased the threonine requirement. Feeding .70% dietary threonine in Exp. 1 maximized (P<.01) gain/feed and minimized (P<:01) plasma urea N. Plasma threonine was increased (P<.01) by threonine supplementat ion and the rate of increase accelerated above .65% d...

Penelitian ini bertujuan untuk mengetahui pengaruh suplementasi l-threonine dalam pakan terhadap berat potong dan non-karkas ayam broiler. telah dilaksanakan di Kampung Bima, Kelurahan Kefa Selatan, Kabupaten Timor Tengah Utara Laboratorium Fakultas Pertanian Universitas Kefamenanu selama 7 minggu, sejak Desember 2019 sampai Januari 2020. menggunakan broiler berumur 14 hari sebanyak 80 ekor. Me...

Driven by consumers’ need to limit gluten intake in their diets, the demand for gluten‐free products continues grow foodservice industry. Two newly released sorghum and millet cultivars were thermally modified responses pre-gelatinization (PG) determined evaluating chemical compositions, amino acids profile functional properties using standard procedures. Protein efficiency ratio, essential aci...

This study was carried out to evaluate the dietary threonine requirement by measuring the plasma free threonine and ammonia concentrations in rainbow trout, Oncorhynchus mykiss after dorsal aorta cannulation. A total of 70 fish (average initial weight 506±8.2 g) were randomly distributed into each of the 14 net cages (5 fish/cage). After 48 hours (h) of feed deprivation, each group was intubate...

A threonine deaminase susceptible to inhibition by isoleucine was purified over 3,000-fold from extracts of Pseudomonas multivorans, a bacterium able to use threonine or alpha-ketobutyrate as sole source of carbon and energy. The enzyme was characterized with respect to molecular weight, dissociation to subunits, and apparent affinities for threonine, isoleucine, and several other ligands. Cert...