AIM Angiotensin-converting enzyme inhibitors (ACEIs) have been shown to block matrix metalloproteinase (MMP)-9 activity, which plays a role in atherogenesis. MMP-9 activity of macrophages is increased during foam cell formation. To investigate the contribution of ACEIs to foam cell formation, we studied the effects of an ACEI, imidaprilat, on THP-1 macrophages and the underlying molecular mecha...

To investigate the effect of acylated ghrelin on the activation of TLR4/NF-κB signaling pathway induced by palmitic acid in human monocyte-derived (THP-1) macrophages, THP-1 macrophages were cultured for 12 h by palmitic acid with various concentrations. The THP-1 macrophages was pretreated by acylated ghrelin at different doses for 4 h before cultivated by palmitic acid (200 μmol/L) for 12 h. ...

Differentiation therapy by induction of tumor cells is an important method in the treatment of hematological cancers such as leukemia. Tumor cell differentiation ends cancer cells' immortality, thus stopping cell growth and proliferation. In our previous study, we found that fucose-containing polysaccharide fraction F3 extracted from Ganoderma lucidum can bring about cytokine secretion and cell...

hepatitis b virus (hbv) dna polymerase transactivated protein 1 (hbvdnaptp1) is a novel protein transactivated by hbv dna polymerase, screened by suppression subtractive hybridization technique (genbank accession no: ay450389). the biological function of hbvdnaptp1 was investigated in this study.we constructed a vector pcdna3.1 (-)/myc-his a-hbvdnaptp1 and used it to transfect acute monocytic l...

in multi-step organic syntheses, the protection and deprotection of tetrahydropyranyl (thp) ethers is one of the most frequently used methods. over the years, different methods have been used from various catalytic systems for the protection of hydroxyl groups as thp ethers and their deprotection. herein we have reported that various alcohols and phenols have been efficiently converted to the c...

Flavonoids are known to exert anti-inflammatory activity but their mechanism at receptor level and comparative potencies less studied. Objective of the study is evaluate, rank order few flavonoids for cytokine inhibitory in monocytes (THP-1) explore signature among toll-like receptors (TLRs). We tested potential inhibit LPS induced cytokines IL-6, TNF alpha human monocyte cell line THP-1 mouse ...

Interleukin-8 (IL-8) or CXCL8 is a potent chemotactic factor that is involved in atherogenesis. IL-8 mediates its pre-inflammatory effects through interaction with CXCR1 and CXCR2. In the present study, we investigated the effects of angiotensin II (Ang II) on IL-8 synthesis and CXCR1/CXCR2 expression of THP-1 monocytes. IL-8 was measured in the culture medium using ELISA. Expression of chemoki...

AIM: To study the effects of α-pinene on nuclear translocation of nuclear factor-κB (NF-κB) and the expression of the inhibitor of NF-κB (IκBα) in human monocyte THP-1 cell line. METHODS: THP-1 cells were incubated with α-pinene (1, 10, and 100 mg/L, for 30 min) before being stimulated with lipopolysaccharide (LPS, 1 mg/L, 30 min). The location of NF-κB p65 subunit (NF-κB/p65) in THP-1 cells wa...

Cobalt protoporphyrin (CoPP) is a potent and effective metalloporphyrin inducer of heme oxygenase-1 (HO-1) activity in many tissues. Here, we report that CoPP induces differentiation of monocytic THP-1 cells into macrophage-like cells. CoPP induced a marked growth inhibition with a slight reduction in viability, and increased adhesion and spreading of THP-1 cells. However, other protoporphyrins...