In this paper, we present a smartphone-readable barcode assay for the qualitative detection of methyl parathion residues, a toxic organophosphorus pesticide that is popularly used in agriculture worldwide. The detection principle is based on the irreversible inhibition of the enzymatic activity of acetylcholinesterase (AchE) by methyl parathion; AchE catalytically hydrolyzes acetylthiocholine i...

The innervation of the anterior segment of the albino rabbit eye was studied with both the histofluorometric technique of Falck for adrenergic fibers and the thiocholine method for cholinergic fibers. Adrenergic fibers were found in heavy concentration in the iris dilator, ciliary processes, and around blood vessels. In lesser number adrenergic fibers were seen in the cornea and filtration area...

A cholinesterase based biosensor was constructed in order to assess the effects of ionizing radiation on exposed AChE. Although the primary objective of the experiment was to investigate the effect of ionizing radiation on the activity of the biosensor, no changes in cholinesterase activity were observed. Current provided by oxidation of thiocholine previously created from acetylthiocholine by ...

Acetylcholinesterase (AChE) terminates nerve-impulse transmission at cholinergic synapses by rapid hydrolysis of the neurotransmitter, acetylcholine. Substrate traffic in AChE involves at least two binding sites, the catalytic and peripheral anionic sites, which have been suggested to be allosterically related and involved in substrate inhibition. Here, we present the crystal structures of Torp...

Acetylcholinesterase and butyrylcholinesterase activities were demonstrated in transverse sections of the rabbit ear artery using a “direct coloring” thiocholine technique. BW 284C5l, iso-OMPA and DFP were used as inhibitors, and acetyl and butyrylthiocholine iodides as substrates. Both enzymes caused stain deposits at the medial-adventitial border of the artery, in a region where sympathetic n...