Abstract Testicular development and function rely on interactions between somatic cells the germline, but similar to other organs, regenerative capacity declines in aging disease. Whether adult testis maintains a reserve progenitor population remains uncertain. Here, we characterize recently identified mouse interstitial expressing transcription factor Tcf21. We found that TCF21 lin are bipoten...

Testicular damage is one of the most hazardous effects as it’s associated with azoospermia. Busulfan (Bu) a highly toxic chemotherapeutic drug that affects testis. Thirty male Swiss albino mice divided into six groups 5 animals each. Control (oral 0.9% saline daily for 75 days); Mel (20 mg/kg/day orally 30 ZnO NPs (5 i.p. BU (single injection 40 mg/kg and then left 45 + dose days followed by 20...

background: spermatogonial stem cells are subpopulation of spermatogonial cells in testis tissue that support beginning and maintenance of spermatogenesis. ubiquitin carboxy-terminal hydrolase l1 (uchl1) could be a specific marker for identification of spermatogonial stem cells including spermatogonial sperm cells (sscs) in testis tissue and during the culture; therefore we undertook this study...

Xenografting of testis tissue from immature males from several mammalian species to immunodeficient mouse hosts results in production of fertilization-competent sperm. However, the efficiency of testis tissue xenografting from adult donors has not been critically evaluated. Testis tissue xenografting from sexually mature animals could provide an option to preserve the genetic material from valu...

Abstract Background Genomic instability is a hallmark of various cancers, and DNA repair an essential process for maintaining genomic integrity. Mammalian cells have developed mechanisms in response to damage. Compared the cellular damage, vivo damage (DDR) specific tissues has not been studied extensively. Objective In this study, mice were exposed whole-body gamma (γ)-irradiation evaluate DDR...

background: busulfan (bus) is a chemotherapy drug that is widely used for cancer treatment. however, administration of busulfan may cause temporary or permanent sterility in male patients. therefore, reduction of this side is necessary. objective: evaluation of the protective effects of l-carnitine and testis homogenized tissue (tht) on sperm parameters and the testis structure after busulfan t...

A determination of the absolute amounts of high-mobility-group proteins 1 and 2 (HMG1 and HMG2) in rat tissues demonstrated that amounts of HMG2 were low in non-proliferating tissues, somewhat higher in proliferating and lymphoid tissues, but were extremely elevated in the testis. This increase was due to a germ-cell-specific form of HMG2 with increased mobility relative to somatic HMG2 on acid...

BACKGROUND It is proved that testis is sensitive to electromagnetic field (EMF) and its damage results in infertility. Exposure to EMF induces reactive oxygen species production and affects on anti-oxidants defense mechanisms. Metallothionein (MT) is a name for a group of low molecular weight (6-7 kDa), sulfhydryl rich proteins. Expression of MT1 and MT2 genes in testis tissue after EMF exposur...