This paper presents a novel microcompartmentalized reaction system for cell-free protein synthesis using semi-permeable microcapsules. We used a microfluidic device to encapsulate template DNA and enzymes for cell-free protein synthesis into semi-permeable microcapsules composed of alginate and polyethylene imine (PEI). The template DNA was encapsulated into individual microcapsules at the conc...

Telomerase adds telomeric repeats to chromosome 3' ends, forestalling the cellular senescence, apoptosis, and genomic instability that result from telomere loss caused by incomplete DNA replication. The telomerase ribonucleoprotein is dedicated to synthesis of tandem, simple-sequence repeats by virtue of its specialization for copying only a specific template region within the integral RNA. Her...

To elucidate the mechanism(s) of influenza viral RNA replication, we have developed an in vitro system in which the templates for viral RNA replication as well as the viral messenger RNAs (mRNAs) are synthesized. Because the synthesis of both the viral mRNAs and the template RNAs occurs in the nucleus of infected cells, we determined whether infected cell nuclei are active in the synthesis of t...

The eukaryotic ribonucleoprotein reverse transcriptase (RT) telomerase uses a template within its integral RNA subunit to extend chromosome ends by synthesis of single-stranded telomeric repeats. Telomerase is adapted to its unique cellular role by an ability to release product DNA in single-stranded form, regenerating free template from the product-template hybrid. Furthermore, by retaining a ...

h-zsm-5 nano-zeolites were synthesized by hydrothermal method using tetrapropylammonium hydroxide (tpaoh) as a template in the presence of various tpabr concentrations. the effect of different tpabr/tpaoh molar ratios was studied on the catalytic performance of dehydration of methanol to dimethyl ether (dme) in a fixed bed reactor under the same operating conditions (t=300°c, p=1 atm, and whsv=...