Legumain (LGMN) is highly expressed in breast cancer (BC) and other solid tumors and is a potential anticancer target. Here we investigate the anti-tumor effects of short hairpin RNAs (shRNAs) targeting LGMN embedded in a microRNA-155 (miR-155) architecture, which is driven by a radiation-inducible chimeric RNA polymerase II (Pol II) promoter. Lentiviral vectors were generated with the chimeric...

acute lymphoblastic leukemia (all) is one of the most prevalent hematologic malignancies in children. although the cure rate of all has improved over the past decades, the most important reason for all treatment failure is multidrug resistance (mdr) phenomenon. the current study aims to explain the mechanisms involved in multidrug resistance of childhood all, and introduces atp-binding cassette...

Myostatin (MSTN) has been shown to be a negative regulator of skeletal muscle development and growth. MSTN dysfunction therefore offers a strategy for promoting animal growth performance in livestock production. In this study, we investigated the possibility of using RNAi-based technology to generate transgenic sheep with a double-muscle phenotype. A shRNA expression cassette targeting sheep MS...

ATP-binding cassette transporter isoform C2 (ABCC2) is exclusively targeted to the apical plasma membrane of polarized cells. Although apical localization of ABCC2 in hepatocytes is crucial for the biliary excretion of a variety of metabolites, the mechanism regulating its apical targeting is poorly understood. In the present study, an apical targeting signal was identified in the first cytopla...

Human induced pluripotent stem cells (iPSCs) bearing monogenic mutations have great potential for modeling disease phenotypes, screening candidate drugs, and cell replacement therapy provided the underlying disease-causing mutation can be corrected. Here, we report a homologous recombination-based approach to precisely correct the sickle cell disease (SCD) mutation in patient-derived iPSCs with...

background: staphylococcus aureus can cause infections with a wide spectrum of illnesses ranging from benign skin infections to bloodstream infection leading to mortality. antimicrobial resistance especially methicillin resistance in s. aureus (mrsa strains) is currently problematic. the emergence of mrsa infections has developed in both the healthcare and the community settings.  the aim of th...