Classical swine fever (CSF) is a contagious and devastating viral disease, causing serious losses in the pig industry worldwide. In the control programmes of CSF, rapid detection and identification of the causative agent is a crucial step. Various PCR based techniques like nested RT-PCR, SYBRGreen based RealTime PCR and TaqMan based Real-time PCR were used for detection of CSFV nucleic acid in ...

background legionellaceae contains legionella genus with over 52 species and 64 serogroups. it is one of the most important causes of respiratory disease in human. more than 30% of hospital-acquired pneumonia is caused by legionella. ventilator-associated pneumonia (vap) is an infection acquired in hospital wards, particularly in intensive care unit (icu). this disease approximately affects 9% ...

Süt ve süt ürünleri içerdikleri yüksek besin değeriyle günlük diyetin önemli bir parçasını oluşturmaktadır. Ancak bu özelliklerinin yanı sıra en fazla hile yapılan gıdalar arasında yer almaktadır. Koyun-keçi sütü ürünlerine inek sütünün karıştırılması ürünlerinde sık karşılaşılan hilelerin başında gelmektedir. Bu durum tüketicileri yalnız ekonomik kayba uğratmakla kalmamakta, aynı zamanda halk ...

Four quantitative reverse transcription-PCR (RT-PCR) methods were compared to evaluate the time course of mRNA formation and decay. Mouse fibroblasts (NIH 3T3) transfected with the human beta-globin open reading frame/c-myc 3'-untranslated region chimeric gene under control of the c-fos promoter (fos-glo-myc) were used for serum-inducible transcription. The amount of fos-glo-myc mRNA, relative ...

Mycoplasma gallisepticum (MG) and Mycoplasma synoviae (MS) are the most pathogenic and economically important mycoplasma pathogens that infect chickens. The development of rapid and innovative molecular diagnostic techniques is of pivotal importance for their effective control. The aim of the present study was to develop a novel duplex TaqMan real-time PCR assay for the simultaneous detection o...