tains more nitrogen than any other element. Fourth, nitrogen is a very dynamic element in the soil system. Its concentration is constantly changing, usually decreasing. The other essential elements are significantly more stable in soils. Nitrogen, therefore, must be routinely added to turfgrass to maintain a soil level sufficient for turfgrass growth. An ideal nitrogen fertilization maintenance...

A recombinant chimeric plasminogen activator (GHRP-scu-PA-32K), consisting of the tetrapeptide Gly-His-Arg-Pro fused to the N-terminus of the low-molecular single-chain urokinase-type plasminogen activator (Leu144^Leu411), was produced by expression in CHO cells. The stable expression cell line was selected for large-scale expression. The product was purified by antibody^Sepharose affinity chro...

Synonymous codon usage (SCU) varies widely among human genes. In particular, genes involved in different functional categories display a distinct codon usage, which was interpreted as evidence that SCU is adaptively constrained to optimize translation efficiency in distinct cellular states. We demonstrate here that SCU is not driven by constraints on tRNA abundance, but by large-scale variation...

Single-chain urokinase-type plasminogen activator (scu-PA) possesses enzymatic activity that increases by 2-3 orders of magnitude upon binding to its cellular cofactor, the u-PA receptor (u-PAR), hence activating an enzymatic cascade initially composed of zymogens. The present study demonstrates that plasminogen activator inhibitor type 3 (PAI-3) reversibly inhibits scu-PA in solution, maintain...

Single-chain urokinase plasminogen activator (scu-PA) that had been modified with N-succinimidyl-3-(2-pyridyldithio)propionate was covalently linked by disulfide bonds to the Fab' of a monoclonal antibody specific for the beta-chain of fibrin (antibody 59D8). scu-PA-59D8 Fab' conjugate was separated from free scu-PA and two-chain urokinase coupled to 59D8 Fab' by two-step affinity chromatograph...

A nanolayer of composite and Rhodococcus erythropolis biocatalyst was studied for the first time for desulfurization of dibenzothiophene as a model sulfur compound and its performance was compared with that of composite and R. erythropolis alone. The nanolayer of composite was synthesized by sol-gel method from ferrous oxalate and zinc oxalate precursors coated on glass by spin coating techniqu...

Abstract The characteristics of peat soil with high organic and moisture content, also compressibility can cause structural failure due to excessive settlement. Improvement settlement parameters be conducted using calcite precipitation method soybean as bio-catalyst, namely Soybean Crude Urease-Calcite Precipitation (SCU-CP). SCU-CP is one the grouting methods improve consistency so that bearin...

The purpose of this study was to prepare, optimize, and characterize a cationic lipid nanoparticle (CLN) system containing multicomponent drugs using a molecular dynamics model as a novel method of evaluating formulations. Puerarin (PUE) and scutellarin (SCU) were used as model drugs. CLNs were successfully prepared using melt-emulsion ultrasonication and low temperature-solidification techniqu...

BACKGROUND An enriched environment for residents with dementia may have a positive effect on the rest-activity rhythm. A small scaled homelike special care unit might be such an enriched environment. The present study shows whether the rest-activity rhythm of residents with moderate to severe dementia responds positively to a transfer from a regular Special Care Unit (SCU) to a small scaled hom...