Subtilisin BPN' has been alkylated using benzyloxycarbonyl-glycylglycyl[1-13C]phenylalanylchloromethane+ ++. Using difference 13C-NMR spectroscopy a single signal due to the 13C-enriched alpha-methylene carbon of the subtilisin-(chloromethane inhibitor) derivative was detected. No evidence for the denaturation/ autolysis of this derivative was obtained from pH 3.5 to 11.5. However, incubating a...

Stabilizing mutations and calcium-dependent stability of subtilisin. Title Stabilizing mutations and calcium-dependent stability of subtilisin. Publication Type Journal Article Year of Publication 2001 Authors Alexander, PA, Ruan, B, Strausberg, SL, Bryan, PN Journal Biochemistry Volume 40 Issue 35 Pagination 10640-4 Date Published 2001 Sep 4 ISSN 0006-2960

Main chain assignments are described for a 266-residue subtilisin mutant, Sbt70, in its 35 kDa complex with an N-terminal prodomain. The assignments provide the basis for understanding how the prodomain assists folding of subtilisin at a residue-specific level.

Silkworm antitrypsin (sw-AT) isolated from larval hemolymph was limitedly digested by Achromobacter lysylendopeptidase, alpha-chymotrypsin, subtilisin BPN', subtilisin Carlsberg, papain, or Pseudomonas elastase. Each proteinase could cleave specific site(s) around the reactive site identified for the reaction of sw-AT and bovine trypsin. Among these proteinases, only subtilisin BPN' was inhibit...

Proteinase specificity of a proteinaceous inhibitor of subtilisin (SSI; Streptomyces subtilisin inhibitor) can be altered so as to strongly inhibit trypsin simply by replacing P1 methionine with lysine (with or without concomitant change of the P4 residue) through site-directed mutagenesis. Now the crystal structure of one such engineered SSI (P1 methionine converted to lysine and P4 methionine...

................................................................................................. 3 Acknowledgments ................................................................................. 5 Introduction ............................................................................. 7 I. The Crystal Structure of Subtilisin Carlsberg in Anhydrous Dioxane and Its Comparison with Those in W...

The activities of Carlsberg and Novo subtilisins towards a number of N-acetylamino acid esters and amino acid esters have been examined. The enzymes were also compared with respect to their efficiency in catalyzing aminblysis reactions, their rates of inactivation by certain aromatic sulfonyl halides, and the rates of deacylation of their N-frans-cinnamoyl derivatives. The enzymes were found to...

An inhibitor of barley malt :t-amylase 2 isolated from barley malt and a specific inhibitor of subtilisin isolated from barley have been sequenced by automatic Edman degradation. The sequence of the first 40 N-terminal amino acids is the same for both proteins. The sequence is: Ala-Asp-Pro-Pro-Pro-Val-His-Asp-Thr-Asp-Gly-HisGlu-Leu-Arg-Ala-Asp-Ala-Asn-Tyr-Tyr-Val-Leu-Ser-Ala-Asn-Arg-Ala-His-Gly...