The substrate specificity of fumarase has been studied by measuring the ability of the enzyme to hydrate or dehydrate derivatives of fumarate and L-malate. Fumarase was observed to hydrate fumarate and its derivatives in the order, fluorofumarate > fumarate > chlorofumarate > bromofumarate > acetylenedicarboxylate > iodofumarate > mesaconate. With the exception of fluorofumarate, water was adde...

Purified soybean lipoxidase was used to test the substrate specificity of all cis,ck-methylene-interrupted isomers of linoleic acid. The natural 9, U-isomer was found to be the best substrate, and the 13,16-isomer 50% as effective. The presence of calcium ions broadened the pattern of specificity. The rates of reaction of a series of polyunsaturated acids in which chain length, number of double...

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