1-Hydroxybenzotriazole, violuric acid, and N-hydroxyacetanilide are three N-OH compounds capable of mediating a range of laccase-catalyzed biotransformations, such as paper pulp delignification and degradation of polycyclic hydrocarbons. The mechanism of their enzymatic oxidation was studied with seven fungal laccases. The oxidation had a bell-shaped pH-activity profile with an optimal pH rangi...

Molybdenum disilicide (MoSi 2 ) is one of the most heat and oxidation resistant materials used in high-temperature applications. In this work, feasibility forming an oxidation-resistant MoSi coating using a low-temperature Sn-Si bath by hot dipping method was evaluated. It found that smooth dense layer can be successfully synthesized on surface Mo-substrate at 700 °C to 1000 °C. Using method, m...

the purpose of present study was to examine the effect of caffeine consumption on substrate metabolism and time to exhaustion during exercise and one hour after exercise in athletic female. eleven female athlete students (age 23±1.54 years, body weight 56.09±5.99 kg, and aerobic power 40.21±1.01ml/kg/min) volunteered and were randomly divided into three groups of control,placebo (starch capsule...

Little information is available on energy metabolism during exercise in girls, particularly the contribution of exogenous carbohydrate (CHO(exo)). The purpose of this study was to determine substrate utilization during exercise with and without CHO(exo) intake in healthy girls. Twelve-yr-old preadolescent (YG; n = 12) and 14-yr-old adolescent (OG; n = 10) girls consumed flavored water (WT) or (...

The protein engineering of CYP enzymes for structure-activity studies and the oxidation of unnatural substrates for biotechnological applications will be greatly facilitated by the availability of functional, whole-cell systems for substrate oxidation. We report the construction of a tricistronic plasmid that expresses the CYP101 monooxygenase from Pseudomonas putida, and its physiological elec...

Cytokinins form a diverse class of compounds that are essential for plant growth. Cytokinin dehydrogenase has a major role in the control of the levels of these plant hormones by catalysing their irreversible oxidation. The crystal structure of Zea mays cytokinin dehydrogenase displays the same two-domain topology of the flavoenzymes of the vanillyl-alcohol oxidase family but its active site ca...

MCrAlY coatings are deposited onto superalloys to provide oxidation and corrosion protection at high temperature by the formation of a thermally grown oxide scale. In this project, the oxidation behaviour of a HVOF CoNiCrAlYSi coating on IN792 was studied for both isothermal oxidation (900, 1000 and 1100 °C) and thermal cycling (100-1100 °C). The microstructural evolution of the CoNiCrAlYSi coa...

Activation energies for partial reactions involved in oxidation of quinol by the bc(1) complex were independent of pH in the range 5. 5-8.9. Formation of enzyme-substrate complex required two substrates, ubihydroquinone binding from the lipid phase and the extrinsic domain of the iron-sulfur protein. The activation energy for ubihydroquinone oxidation was independent of the concentration of eit...

Microbial phenol oxidases and peroxidases mediate biogeochemical processes in soils, including microbial acquisition of carbon and nitrogen, lignin degradation, carbon mineralization and sequestration, and dissolved organic carbon export. Measuring oxidative enzyme activities in soils is more problematic than assaying hydrolytic enzyme activities because of the non-specific, free radical nature...