There are contradictory observations about the different radiosensitivities of cancer stem cells and cancer non-stem cells. To resolve these contradictory observations, we studied radiosensitivities by employing breast cancer stem cell (CSC)-like MDA-MB231 and MDA-MB453 cells as well as their corresponding non-stem cells. CSC-like cells proliferate without differentiating and have characteristi...

Activated protein C is a plasma anticoagulant. For activated protein C to function as an anticoagulant, it must form a complex with protein S. Protein S anticoagulant activity is neutralized by formation of a reversible complex with C4b binding protein (C4bBP). C4bBP is an acute-phase plasma protein. When C4bBP levels increase, mass action forces the level of free protein S to decrease, giving ...

It is generally agreed that survival of mice after whole-body irradiation depends on the restoration of hemopoietic function. This restoration may result from the proliferative activity of cells that have survived the radiation, or of cells from unirradiated donors that have been transplanted into the irradiated animals. Recently, we have suggested that such hemopoietic restoration depends in l...

The fundamentals of Inherently Safer Design were not fully appreciated in the initial design (or re-design) in the following series of case histories. Two case histories involving the basic element of plant layout to minimize property damages and injury will be covered first. Simple physical separation could have reduced the losses. A case history that occurred in a bulk chemical terminal tank ...

Proton beam irradiation is a form of advanced radiotherapy providing superior distributions of a low LET radiation dose relative to that of photon therapy for the treatment of cancer. Even though this clinical treatment has been developing for several decades, the proton radiobiology critical to the optimization of proton radiotherapy is far from being understood. Proteomic changes were analyze...

Historically it has been shown repeatedly that single high doses of radiation do not allow a therapeutic differential between tumor and critical normal tissues but dose fractionation does. The purpose of conventional dose fractionation is to increase dose to the tumor while preserving normal tissue function. Tumors are generally irradiated with 2Gy dose per fraction delivered daily to a more or...

In vitro survival measurements using two human head-and-neck cancer (HNC) cell lines were performed. The specially designed split-dose surviving fraction was obtained and fitted to the linear-quadratic formalism. The repair halftime (Tr), the potential doubling time (Td), a/β and radiosensitivity a, were estimated. Other radiobiological models: EUD, BED, TCP, etc., were used to examine the pote...

PURPOSE To investigate the radiosensitivity of uveal melanoma cell lines by a clonogenic survival assay, to improve the efficiency of the radiation regimen. METHODS Four primary and four metastatic human uveal melanoma cell lines were cultured in the presence of conditioned medium. After single-dose irradiation (0-12 Gy), colonies were allowed to form for 6 to 14 days. Two cutaneous melanomas...

Exponentially growing cells and cells that were allowed to enter a plateau or nondividing state of growth by depleting the medium of growth-essential nutrients were used in these studies. Nondividing Chinese hamster ovary cells in vitro are less sensitive to actinomycin D (AMD) than are exponentially growing cells. The cells were tested for their ability to recover from AMD-induced potentially ...