Interspecies antigenic determinants have been used as markers to study the evolution of oncoviruses. By radioimmunologic techniques, it has been possible to detect crossreactivities among the major structural proteins of prototype viruses representing each of the three major Oncovirinae genera. Two different subsets of interspecies antigenic determinants were demonstrated. One encompasses known...

Alphavirus-based replicon systems are frequently used as preclinical vectors and as antigen discovery tools, and they have recently been assessed in clinical vaccine trials. Typically, alphavirus replicon RNAs are delivered within virus-like replicon particles (VRP) that are produced following transfection of replicon RNA and two helper RNAs into permissive cells in vitro. The non-structural pr...

During the past decade, the Protein Structure Initiative (PSI) centres have become major contributors of new families, superfamilies and folds to the Structural Classification of Proteins (SCOP) database. The PSI results have increased the diversity of protein structural space and accelerated our understanding of it. This review article surveys a selection of protein structures determined by th...

objective: the aim of the present study was the production of recombinant lentviruses that express mir-16. after transduction, altered expression levels of mirna and its target protein were analyzed. methods: a dna fragment that contained the mir-16 precursor was cloned in a lentiviral plasmid. lentiviral vector particles were produced by transient calcium phosphate co-transfection of 293t cell...

the impact of pgf2? on total proteins, free amino acids and the amino transferases in the gonads of silkworm, bombyx mori l. were investigated in the present experiment. the effect of pgf2? resulted in increase in the total protein and free amino acid contents in the gonads of silkworm larvae indicating improved structural and dynamic levels of organization. the enhanced activity levels of amin...

background: candida albicans is one of the most important opportunistic pathogens that suppress immunologic mechanisms of the host. it is speculated that structural and secretory proteins of c. albicans have immunomodulatory effects in cancer. objective: to evaluate the effects of c. albicans structural and secreted proteins on intratumoral cd4/cd8 ratio as well as the survival rate in balb/c t...

Currently, there is a major effort to map protein-protein interactions on a genome-wide scale. The utility of the resulting interaction networks will depend on the reliability of the experimental methods and the coverage of the approaches. Known macromolecular complexes provide a defined and objective set of protein interactions with which to compare biochemical and genetic data for validation....