Beta-catenin is an ubiquitously expressed cytoplasmic protein that has a crucial role in both E-cadherin-mediated cell-cell adhesion and as a downstream signaling molecule in the wingless pathway. Stabilization of beta-catenin followed by nuclear translocation and subsequent T-cell factor/lymphoid-enhancing factor-mediated transcriptional activation has been proposed as an important step in onc...

Progression to androgen-independent growth of human prostate cancers may be mediated by alterations in the structure and/or expression of the androgen receptor (AR) gene. To date, mutations in the AR gene have largely been identified in hormone refractory tumors. In this study, single-strand conformational polymorphism analysis and DNA sequencing of the entire AR gene coding region was performe...

Mutation of the p53 tumor suppressor gene frequently occurs in a variety of tumors including lung, breast, gastrointestinal, and brain, as well as lymphomas-leukemias. Neuroblastoma, one of the most common solid tumors in childhood, often has amplification of the N-myc gene. We examined for mutations of the p53 tumor suppressor gene by single-strand conformational polymorphism using polymerase ...

dna polymorphism within diacylglycerol transferase 2 (dgat2) / monoacyl glycerol transferases 2 (mogat2), leptin and butyrophilin genes were analysed using pcr-sscp in murrah buffalo. the single strand conformation polymorphism (sscp) analysis of amplified gene fragment in exon 5 of mogat2, exon 3 of leptin and intron 1 of butyrophilin gene revealed different patterns. a, b and c showed the fol...

AIM To identify the disease-causing mutation responsible for the presence of congenital cataract in a Chinese family. METHODS The study recruited a four-generation Chinese pedigree affected by autosomal dominant congenital cataract (ADCC). Family history and the history of cataract extraction were recorded. Blood samples were collected from individuals for DNA extraction. Direct sequencing of...

in the present study, the polymorphism of myostatin gene (mstn) in native chickens of western azerbaijan rearing and breeding institute was investigated. the blood samples were collected from eighty two randomly selected hens. genomic dna was extracted from blood samples and a fragment of myostatin including 599 bp in promoter and exon 1 was amplified using pcr method. breeding values for body ...

The product of the obese (ob) gene, leptin, is a secreted protein that is important in the regulation of body weight. Mice with mutations in the ob gene are obese and diabetic and manifest reduced physical as well as metabolic activity. In this study, we tested the possibility that mutations in the OB gene may contribute to human obesity. We report the isolation and partial sequence of the huma...

background: varicocele is associated with the failure of ipsilateral testicular growth and development, and the symptoms of pain and reduced fertility. the highly condensed structure of the sperm nuclear chromatin is provided by proper expression of transition nuclear protein (tnp) genes, so any dysregulational expression of these genes results in abnormal spermatogenesis and infertility. objec...

cystic fibrosis (cf) is the most common inherited disorder in caucasian populations, with over 1400 cystic fibrosis transmembrane conductance regulator (cftr) mutations. the type of mutations and their distributions varies widely between different countries and/or ethnic groups. seventy iranian cystic fibrosis patients were screened for the cftr gene mutation using arms/pcr (amplification refra...