Purpose: Fusobacterium nucleatum is an opportunistic pathogen involved in periodontal diseases, extraoral infections, and colorectal cancer. necrophorum causes a variety of necrotic infections. F. are classified into five two subspecies, respectively. Conventional identification methods were technically hard to distinguish each subspecies species accurately. The purpose the present study was de...

background: trichomoniasis, caused by trichomonas vaginalis , is one of the most common sexually transmitted infections in the world. diagnosis of t. vaginalis is performed by different methods, including wet mount, culture, serological methods and pcr, which required laboratory equipments and expert laboratory personnel. the aim of this study was evaluation of immunochromatographic strip test ...

Methods A case of oral allergy syndrome to sapodilla and custard apple was investigated following approval by Institutional Ethics Committee. Sapodilla allergy was confirmed by diagnostic tests (SPT and allergen-specific IgE). Sapodilla proteins were separated on SP-Sepharose by adsorption at pH 4 followed by step elution at pH 5 (SP1), and with increasing NaCl – 0.1 M (SP2) and 0.2 M (SP3). Fo...

Cholera outbreak is more common in developing countries. The causative agent of the disease is Vibrio cholerae strains O1 and O139. Traditional diagnostic testing for Vibrio is not always reliable, because Vibrio can enter a viable but non cultivable state. Therefore, nucleic acid-based tests have emerged as a useful alternative to traditional enrichment testing.       In this investigation, a...

A very sensitive and specific method for the random amplification of whole DNA molecules and genomes ranging from 400 base pairs (bp) to 40 Megabase (Mb) is described. This simple, two step PCR (1-3) strategy utilizes tagged random primers that consist of a pool of all possible 3' sequences for binding to the target DNA and a constant 5' region for the detection of incorporated primers. As litt...

background: among the most important parasitic disease, causing diarrhea, gi­ardia lamblia is noteworthy. nowadays detection methods for these parasites in­clude parasitological methods such as microscopic examination. the sensitivity of these methods relies on the expertise and experience of examiners. in contrast, molecular methods such as pcr are less dependent on the expertise of the exam­i...

A fast and simple method to detect mycoplasmal contamination in simulated samples of animal sera by using a PCR was developed. The following five mycoplasma species that are major cell culture contaminants belonging to the class Mollicutes were investigated: Mycoplasma arginini, Acholeplasma laidlawii, Mycoplasma hyorhinis, Mycoplasma orale, and Mycoplasma fermentans. After a concentration step...

In the present study a protocol of in situ reverse transcriptase-nested polymerase chain reaction (in situ RT-nested PCR) was examined based on the following modifications. (i) To exclude false positive signals caused by "DNA repair mechanisms" and "endogenous priming", a two-step PCR was applied after reverse transcription. The first step was performed in the presence of extrinsic primers and ...

Recently, the applications of nanomaterial-assisted polymerase chain reaction (nanoPCR) have received considerable attention. Several potential mechanisms have been proposed, but mainly according to the results of PCR assays under specific conditions and lacking direct and general evidence. The mechanism of nanoPCR has not been elucidated yet. Here, taking gold nanoparticles (AuNPs) as an examp...