A novel stability-indicating reversed-phase high-performance liquid chromatographic (HPLC) method has been developed for the quantitative determination of darunavir ethanolate, an HIV-1 protease inhibitor. The chromatographic separation was achieved using an X-Bridge C18 (150 × 4.6 mm × 3.5 µm) HPLC column in isocratic mode employing 0.01M ammonium formate (pH.3.0) buffer and acetonitrile in th...

A sensitive, stability-indicating liquid-chromatographic method for analysis of tamsulosin in the presence of its degradation products has been developed and validated. Efficient chromatographic separation was achieved on a C18 column with a simple isocratic mobile phase consisting methanol: 50 mM phosphate buffer (pH 7.8, ratio 80:20) at a flow rate of 1 ml/m. The linearity of the method was e...

The present paper describes stability indicating reverse phase Ultra performance liquid chromatographic (RPLC) assay method for Atrovastatin Calcium in bulk drugs. The developed method is also applicable for the related substance determination and degradation products generated from forced decomposition studies. The drug substance was subjected to stress conditions of acid hydrolysis, base hydr...

A stability-indicating liquid chromatographic (LC) method was developed for the determination of posaconazole in bulk. Chromatographic separation was achieved using an isocratic elution in a reversed-phase system, with a mobile phase composed of methanol-water (75:25, v/v), at 1.0 mL min(-1) flow. Samples were exposed to degradation under thermal, oxidative and acid/basic conditions, and no int...