Members of the Trypanosomatidae were studied for their ability to acquire host IgG through a possible Fc receptor. A simple rosette test was devised in which the different species and forms of protozoa were mixed with SRBC sensitized with subagglutinating does of IgG, IgM, and F (ab') 2 anti-SRBC, and the pelleted mixture was observed for the number of clumps (rosettes) formed between the paras...

Mice injected intravenously with sheep erythrocytes (sRBC) demonstrate a transient increase in splenic cAMP levels (4-fold), which peak at 2 min after injection and return to basal levels by 20 min. In addition to the change in cAMP, an increase in splenic cGMP levels (1.5-fold) occurs beginning 5-10 min after sRBC injection, and persists for up to 7 days. During this period cAMP levels remain ...

BACKGROUND A major problem in cancer chemotherapy is the existence of primary resistance and/or the acquisition of secondary resistance. Many cellular defects contribute to chemoresistance, but epigenetic changes can also be a cause. METHODS A DNA methylation microarray was used to identify epigenetic differences in oxaliplatin-sensitive and -resistant colorectal cancer cells. The candidate g...

Graczyk S. , A. Wiel iczko, A. Pl iszczak-Król , B. Janaczyk: Humoral and Cellular Response of Pheasants Vaccinated against Newcastle Disease and Haemorrhagic Enteritis. Acta Vet Brno 75, 2006: 379-386. The purpose of the experiment was to define whether and to what extent can prophylactic vaccinations against Newcastle disease (ND) and haemorrhagic enteritis (HE) affect the humoral and cellula...

Six congenic lines containing B complex recombinants R1 = B-F/B-L24, B-G23; R2 = B-F/B-L2, BG23; R3 = B-F/B-L2, B-G23; R4 = B-F/B-L2, B-G23; R5 = B-F/B-L21, B-G19; and R6R6 = B-F/B-L21, B-G23 were tested individually for antibody response against SRBC. R2, R3 and R4 arose from independent recombination events but are serologically identical. Each B complex recombinant was crossed to inbred Line...

Chicken peritoneal exudate cells (PEC) were induced by Sephadex G-50 injection and were used for studying their in vitro phagocytic activity towards sheep red blood cells (SRBC) and Salmonella gallinarum. The peritoneal macrophages-were phagocytic to opsonised as well as to non-opsonised SRBC or S. gallinarum. The recognition and phagocytosis depended on time of exposure as well as the intensit...

Trans-generational antibody transfer constitutes an important mechanism by which mothers may enhance offspring resistance to pathogens. Thus, differential antibody deposition may potentially allow a female to differentiate offspring performance. Here, we examined whether maternal immunization with sheep red blood cells (SRBC) prior to egg laying affects sex-specific yolk antibody transfer and s...

Monoclonal antibodies to sheep erythrocytes (SRBC) have proved useful in identifying two Fc receptors on mouse macrophages, one for IgG2a, and one for IgG1 and IgG2b. We have used monoclonal IgG3 anti-SRBC to identify a third Fc receptor on mouse macrophages which binds IgG3 uniquely. This receptor is present on primary resident and thioglycolate-induced peritoneal macrophages and on some macro...

IntroductionHuman norovirus (HuNoV) is a leading cause of infectious gastroenteritis. Since HuNoV shows resistance to alcohol, chlorine-based sanitizers are applied decontaminate the virus on environmental surfaces. Chlorous acid water (CA) has been recently approved as novel disinfectant categorized Type 2 OTC medicine in Japan. In this study, we aimed evaluate capability CA inactivate HuNoV.M...

Johnson, H. M. (Robert A. Taft Sanitary Engineering Center, Cincinnati, Ohio), K. Brenner, R. Angelotti, and H. E. Hall. Serological studies of types A, B, and E botulinal toxins by passive hemagglutination and bentonite flocculation. J. Bacteriol. 91:967-974. 1966.-Formalinized sheep red blood cells (SRBC), sensitized with types A, B, and E botulinal toxoids and toxins by bis-diazotized benzid...