In dividing cells, the assembly and contraction of the cytokinetic actomyosin ring (CAR) is precisely coordinated with spindle formation and chromosome segregation. Despite having a cell wall, the fission yeast Schizosaccharomyces pombe forms a CAR reminiscent of the structure responsible for the cleavage of cells with flexible boundaries. We used the myo2-gc fission yeast strain in which the c...

The RZZ (Rod, ZW10, and Zwilch) complex and Mad1 proteins tightly associate with kinetochores to generate the spindle checkpoint signal, but they are released when a kinetochore forms mature microtubule attachments. Here we demonstrate that the centromere protein CENP-I is required to generate a stable association of RZZ and Mad1 with kinetochores. CENP-I also inhibits their removal by dynein s...

The organization of microtubules is determined in most cells by a microtubule-organizing center, which nucleates microtubule assembly and anchors their minus ends. In Saccharomyces cerevisiae cells lacking She1, cytoplasmic microtubules detach from the spindle pole body at high rates. Increased rates of detachment depend on dynein activity, supporting previous evidence that She1 inhibits dynein...

itotic spindle positioning involves a con-formational switch in LGN, the mammalian version of the invertebrate Pins protein, duces a spindle rocking that represents the first genetically manipulable model for studying spindle positioning in vertebrates. The switch operates via the opening of LGN, which can normally fold back onto itself. The unfolding is triggered by binding to either membrane-...

itotic spindle positioning involves a con-formational switch in LGN, the mammalian version of the invertebrate Pins protein, duces a spindle rocking that represents the first genetically manipulable model for studying spindle positioning in vertebrates. The switch operates via the opening of LGN, which can normally fold back onto itself. The unfolding is triggered by binding to either membrane-...

Spindle cell hemangioma is a benign vascular tumor presenting as multifocal red or bluish nodules on the distal limbs. We present a 57-year-old woman with five years history of multiple, soft, violaceous nodules on her hands. Pathologic study indicated spindle cell hemangioma.

itotic spindle positioning involves a con-formational switch in LGN, the mammalian version of the invertebrate Pins protein, duces a spindle rocking that represents the first genetically manipulable model for studying spindle positioning in vertebrates. The switch operates via the opening of LGN, which can normally fold back onto itself. The unfolding is triggered by binding to either membrane-...

Due to manufacturing flaws or cyclic loading, cracks frequently appear in a rotating spindle system. These cracks markedly affect the dynamic characteristics in higher modes of the rotating machinery. For faster rotational speeds, especially for super-high-speed cutting, a spindle with magnetic bearings is necessary. However, most investigations into spindle system dynamic characteristics have ...

Background: The aim of this study was to assess effects of cysteamine as an anti-oxidant on rate of in vitro maturation of oocyte and determination of its effects on spindle size and shape. Methods: Pre-mature mice were primed with pregnant mare stimulating gonadotrophin (PMSG) and germinal vesicle (GV) stage oocytes were obtained 48 h after. The oocytes were cultured in tissue culture medium (...