Objectives: The Coronavirus Disease 2019 (COVID-19) caused by SARS-CoV-2 has been the current global pandemic concern. With a high transmission rate, especially through direct contact, this disease spreads from person to person, and in turn led huge number of infections on scale. Methods: In present study, comparative genomic analysis was performed using 151 gene sequences viral spike protein r...

Introduction The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic has been fueled by new variants emerging from circulating strains. Here, we report results a genomic surveillance study of SARS-CoV-2 on Jeju Island, Republic Korea, February 2021 to September 2022. Methods A total 3,585 positive samples were analyzed Sanger sequencing the gene encoding spike protein before p...

objective: the aim of this study was to construct a pcdna3.1+ vector containing fmdv type o/irn/1/2007-vp1 gene, protein expression in bhkt7 cells and evaluation of immune response in balb/c mice. materials and methods: fmdv type o/irn/1/2007 was isolated from a cattle in ray in 2007 and serotyped. the purified vp1 gene was sub-cloned into the ptz57r/t vector and pcdna3.1+ expression vector. t...

Transient gene expression (TGE) in mammalian cells is a method of rapidly generating recombinant protein material for initial characterisation studies that does not require time-consuming processes associated with stable cell line construction. High TGE yields are heavily dependent on efficient delivery plasmid DNA across both the plasma and nuclear membranes. Here, we harness nucleoside diphos...

background and purpose: mycotoxins are secondary fungal metabolites with a very high diversity that are produced by some species of aspergillus which frequently leads to contaminate food and agricultural products. recently, elimination of aflatoxin contamination in food and feed has been considered by scientists worldwide. although, the antibacterial and antifungal effects of vitamins as natura...

sunn pest (eurygaster integriceps put.) is one of the major pests of wheat in iran. identification of resistantcultivars is an effective integrated pest management (ipm) strategy. to identify the genetic response of wheat and triticale to sunn pest, and determine the relationship between resistance and morpho physiological traits, eight bread wheat breeding lines/cultivars, four durum wheat lin...

background: toxoplasmosis is a worldwide-distributed infection which is mostly asymptomatic but can cause serious health problems in congenitally-infected newborns and immunecompromised individuals. research is undergoing both to improve toxoplasma serological tests, which play the main role in laboratory diagnosis of the infection, and develop an effective vaccine to prevent the infection. som...

Coronavirus disease 2019 (Covid-19) is a very serious, contagious, and infectious that cause severe acute respiratory syndrome. It has four structural base proteins: envelope (E), nucleocapsid (N), membrane (M) spike (S) protein which responsible for the viral attachment, fusion, entry [1].