The first successful artificial insemination in cats was described 40 years ago. However, the insemination in cats is at present not as commonly performed in daily veterinary practice as the insemination in dogs due to the practical difficulties in collecting a sperm sample from the tomcat, the small volume of the sperm obtained, the few possibilities to determine the optimal timing of insemina...

Background: Preliminary data has also shown that there is less fragmented sperm in 2nd and 3rd ejaculates compared to first ones which could be a major factor in determining the pregnancy outcome. Assessing this factor objectively and relating it to other parameters in sperm quality in this study could result in new prediction criteria for the pregnancy outcome. Materials and Methods: As one of...

The Sperm Chromatin Structure Assay (SCSA®) is the pioneering sperm DNA fragmentation assay that precisely measures the percent of sperm in a semen sample that have Sperm DNA Damage (SDD), a negative factor for successful pregnancy. The SCSA® is a rapid, dual parameter, computer driven assay with diligent unbiased flow cytometer measurements on five to ten thousand sperm per sample providing ri...

The introduction of the technique of intracytoplasmic sperm injection to achieve fertilization, especially using surgically retrieved testicular or epididymal sperm from men with obstructive or non-obstructive azoospermia, has revolutionized the field of assisted reproduction. The techniques for the retrieval of spermatozoa vary from relatively simple percutaneous sperm aspiration to open excis...

Potential sperm limitation has become a concern for blue crabs Callinectes sapidus in Chesapeake Bay. However, methods to quantify sperm in blue crabs are time intensive, which limits the sample size that can be obtained for sperm limitation studies. Our goal was to develop an efficient method for rapidly preparing spermathecae samples that more quickly quantifies sperm in blue crabs. Ultimatel...

This study of male reproductive health in the Czech Republic resulted from community concern about potential adverse effects of air pollution. We compared young men (18 years of age) living in Teplice, a highly industrialized district with seasonally elevated levels of air pollution, to those from Prachatice, a rural district with relatively clean air. Surveys were scheduled for either late win...

Assisted reproductive techniques (ART) have now been extensively incorporated in the management of infertile couples. But even after rapid methodological and technological advances the success rates of these procedures have been below expectations. This has led to development of many sperm preparation protocols to obtain an ideal semen sample for artificial reproduction. Sperm apoptosis has bee...

It is universally recognized that cryopreservation impairs sperm quality. In order to improve postthawing sperm survival and motility, media of different composition and different protocols have been proposed. However, no clear evidence is available to understand which are the most efficient protocol and medium for sperm cryopreservation. The present study evaluates the efficiency of two differ...

Received: 21 January, 2014 Accepted: 30 March, 2014 Published: 20 April, 2014 A study was conducted to investigate the effect Gonadotropins supplied to culture medium on human sperm parameters and sperm DNA structure during in vitro sperm activation. Thirty infertile patients were included. Semen sample was divided into 3 aliquots. One mL of SMART medium either alone (control group) or supplied...