Treatment of male rats with ornidazole results in reversible infertility, which is associated with the detection of the contraception-associated protein 1 (CAP1) in epididymal fluid. The protein, which is present in sperm but not detectable in epididymal fluid of fertile rats, seems to be shed from sperm during ornidazole administration. Cloning and characterization of the gene revealed a high ...

BACKGROUND The objectives of this study were (i) to evaluate whether the combined use of Syto 16 and 7-amino-actinomycin-D (7-AAD) allows the detection of sperm apoptosis and (ii) to describe a new multiparameter flow cytometric method to assess simultaneously sperm concentration (SC), viability and apoptosis as well as leukocyte concentration. METHODS Semen samples from 68 patients were eval...

It was reported that M1 monoclonal antibody (M1 mab) inhibited hamster sperm-egg fusion in a dose-dependent manner. The antigen was localized to the equatorial segment (ES) of the hamster sperm, and that after the acrosome reaction, it was exposed on the surface of the sperm plasma membrane overlying the ES. The M1 antigen therefore fulfilled the main requirements of a sperm component that migh...

BACKGROUND A key role of estrogens in human sperm biology has been recently suggested by aromatase and estrogen receptor detection in human testicular germ cells and ejaculated spermatozoa. However, the involvement of these hormones in the sperm maturation process is still not defined. The aim of this work was to investigate the expression of estrogen receptors, ER-alpha and ER-beta, in human e...

introduction: membrane cofactor protein (mcp or cd46) is one of the regulatory of complement system. it is present in all somatic cells except in rbc. it is also present in all body fluids and semen. the aim of this study was measuring the concentration of this protein in semen of fertile and infertile individuals and to study the relationship between the concentration of this protein and semen...

Background: Sperm DNA is susceptible to oxidative damage due to intrinsic and extrinsic factors which cause oxidative stress and due to limited DNA damage detection and repair mechanism. Reactive oxygen species (ROS) are the chief cause of sperm DNA damage. So, this study was planned to assess oxidative stress levels and correlate with sperm DNA damage. Material and Method: The study included 3...

OBJECTIVE To determine which genetic tests are being performed on sperm donor applicants in the United States. DESIGN An electronic survey was distributed to 26 sperm banks to evaluate their genetic testing practices. SETTING Sperm banks in the United States. PATIENT(S) Not applicable. INTERVENTION(S) None. Survey of current practices. MAIN OUTCOME MEASURE(S) Survey of current practic...

The identification of semen is of paramount importance in the investigation of rape and other crimes involving sexual assault. The most commonly used procedures for semen identification center on the detection of sperm o r the detection of prostatic acid phosphatase activity; methods involving the detection of spermine, choline, or semen antigens are less commonly employed. Unfortunately, none ...

BACKGROUND Previous researches about the effect of smoking on semen quality are contradictory, and the mechanism behind the harmful effect of smoking on semen quality still remains unclear until today. OBJECTIVE The objectives of this study are evaluation of the relationship between smoking and fertility, investigation of the effects of cigarette smoking on sperm parameters and detection of p...

The objective of this study was to determine the effects of method and clinician on stallion sperm morphology evaluation. Five clinicians evaluated 60 semen samples using wet-mount preparations with phase-contrast, eosin/nigrosin-stained semen smears, and Papanicolaou-stained semen smears. There were significant differences among methods for all sperm morphology categories and most intra-class ...