Here we describe an improved method of separating sperm cells from lysed epithelial cells via acoustic cell trapping. In particular, we show that in the processing of samples with low numbers of sperm cells, addition of polymeric beads to the sample[1] allowed for better performance in terms of higher trapping efficiency versus our previous acoustic differential extraction (ADE) results[2]. We ...

This report focuses on the development of a method for chemically induced enhancement of cell elution and recovery from cotton swabs. The method exploits the exclusive use of detergents for intact cell removal, and can be utilized in conjunction with, or to circumvent, conventional differential extraction (DE). Samples treated with Sarkosyl (54.4 +/- 1.8%) and sodium dodecyl sulfate (SDS) (78.5...

Purpose: to study the influence of level active forms oxygen in native spermatozoa roosters on qualitative indicators freshly exposed and deconed individual ejaculators (mobility, damage cell membranes sperm) determining permissible AFC generation improve cryoponement protocol. Materials methods. The object was Rodi-Aland Red (n = 20) breed at age 44 weeks life. All males were kept cells with “...

This study was performed to evaluate the effectiveness of three different conditions of osmotic pressure (325, 365 and 385 mOsm kg-1) in combination with dimethyl sulfoxide (DMSO) and NaCl or glucose on spermatozoa DNA damage, rate and duration of motility. Sperm was collected from eight healthy mature Mesopotamian catfish, evaluated microscopically and pooled at 25 °C. The pooled sperm samples...

Sperm competition theory predicts that when males are certain of sperm competition, they should decrease sperm investment in matings with an increasing number of competing ejaculates. How males should allocate sperm when competing with differently sized ejaculates, however, has not yet been examined. Here, we report the outcomes of two models assuming variation in males' sperm reserves and male...

Certain sperm components labeled with fluorescein isothiocyanate or its radioactive derivative, 125I-diiodofluorescein isothiocyanate (125IFC), are transferred at fertilization to the egg, where they persist throughout early cleavage stages at a localized site in the embryo cytoplasm (Gabel, C. A., E. M. Eddy, and B. M. Shapiro, 1979, Cell, 18:207-215; Gundersen, G. G., C. A. Gabel, and B. M. S...

Remarkably, mammalian sperm maintain a substantive proportion of their progressive swimming speed within highly viscous fluids, including those of the female reproductive tract. Here, we analyse the digital microscopy of a human sperm swimming in a highly viscous, weakly elastic mucus analogue. We exploit principal component analysis to simplify its flagellar beat pattern, from which boundary e...

objective: epididymo-orchitis (eo) potentially results in reduced fertility in up to 60% of affected patients. the anti-inflammatory effects of korean red ginseng (krg) and its ability to act as an immunoenhancer in parallel with the beneficial effects of this ancient herbal medicine on the reproductive systems of animals and humans led us to evaluate its protective effects against acute eo. ma...

ABSTRACT In mammals, sperm-borne regulators can be transferred to oocytes during fertilization and have different effects on the formation of pronuclei, first cleavage zygotes, development preimplantation embryos even metabolism individuals after birth. The regulatory role sperm microRNAs (miRNAs) in bovine has not been reported detail. By constructing screening miRNA expression libraries, we f...