نتایج جستجو برای: specific vans1 primer

تعداد نتایج: 1061859  

2009
Cheng-Hong Yang

Before performing polymerase chain reactions (PCR), a feasible primer set is required. Many primer design methods have been proposed for design a feasible primer set. However, the majority of these methods require a relatively long time to obtain an optimal solution since large quantities of template DNA need to be analyzed. Furthermore, the designed primer sets usually do not provide a specifi...

ژورنال: کومش 2019
Hashemi , Atieh , Mohammadgholizad , Fatemeh ,

Introduction: Epithelial cell adhesion molecule (EpCAM) is highly expressed on epithelial tumors. So, EpCAM is a valuable antigen for targeted therapy. Using monoclonal antibodies (mabs) is an attractive approach for targeted cancer therapy. Importantly, limitations of intact mabs including large size led to the development of antibody fragments such as single chain fragment variable (scfv). Pi...

Journal: :Journal of the Japan Society of Colour Material 1999

Journal: :Genome research 1996
S H Hardin L B Jones R Homayouni J C McCollum

This paper describes a novel method of primer walking using octamer oligonucleotides to prime DNA sequencing reactions. Octamer sequencing is compatible with isotopic and fluorescent sequencing chemistry, reaction conditions are optimized such that the samples can be processed in parallel, and the procedure has the potential to be automated. This strategy is faster than the traditional primer w...

Journal: :Forensic science international 2003
Craig Leibelt Bruce Budowle Patrick Collins Yasser Daoudi Tamyra Moretti Gary Nunn Dennis Reeder Rhonda Roby

A population study of Chamorros and Filipinos using short tandem repeat (STR) loci amplified with the AmpFlSTR Profiler Plus PCR amplification kit demonstrated an excess of observed homozygosity at the D8S1179 locus. Use of a different set of D8S1179 primers to type the same samples did not demonstrate an excess of homozygosity and showed discordant genotypes at the D8S1179 locus. A single poin...

Journal: :Genetics and molecular research : GMR 2012
M J Wang H D Zou Z S Lin Y Wu X Chen Y P Yuan

We developed EST-PCR markers specific to barley chromosome 2H, for the purpose of effectively tracing alien chromosomes or chromosome parts in the wheat genetic background. The target alien chromosome 2H confers high resistance to pre-harvest sprouting, which is a worldwide natural disaster in wheat. A total of 120 primer pairs were selected by combining the wheat group 2 chromosomes of th...

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