We describe here an approach for the isolation and characterization of substrate attached membranes of cultured cells. The procedure for ventral membrane preparation is based on a short incubation with ZnCl,, followed by shearing with a stream of buffer. By varying the intensity of shearing it was possible to obtain reproducibly either entire ventral membranes or highly enriched focal contacts....

Differential phase-contrast imaging with hard x rays can have important applications in medicine, material sciences, and energy research. Phase-contrast methods based on microperiodic optics, such as shearing interferometry, are particularly attractive because they allow the use of conventional x-ray tubes. To enable shearing interferometry with x rays up to 100?keV, we propose using grazing-in...

Shearing stress of wheat stalk was measured for four moisture content levels (15, 25, 35 and 45%, w.b.), three cutting heights (100, 200 and 300 mm), two types of cutting knives, smooth and serrated edge and three blades oblique angle (0, 15 and 30 deg.). The results of data analysis showed that the shearing stress of wheat stems decreased as the moisture content decreased. The shearing force o...

Mid-pregnancy shearing has been promoted in New Zealand as a technique to improve both lamb birthweights and survival. In the literature mid-pregnancy shearing has resulted in an increase lamb birthweight. However, the results have been inconsistent in magnitude and birthrank specificity. Additionally the mechanism responsible for the birthweight response has not been identified. The objectives...

The extent to which angular momentum transport in accretion discs is primarily local or non-local and what determines this is an important avenue of study for understanding accretion engines. Taking a step along this path, we analyze simulations of the magnetorotational instability (MRI) by calculating energy and stress power spectra in stratified isothermal shearing box simulations in several ...

Dry adhesion observed in the feet of various small creatures has attracted considerable attention owing to the unique advantages such as self-cleaning, adaptability to rough surfaces along with repeatable and reversible adhesiveness. Among these advantages, for practical applications, proper detachability is critical for dry adhesives with artificial microstructures. In this study, we present a...

In a previous experimental study, it was observed that the break-through process of a cleavage front across a high-angle grain boundary can be highly nonuniform. While the central part of the boundary can be cleaved quite smoothly, the rest parts must be sheared apart. In this paper, the trapping effect of grain boundary shearing is analyzed in considerable detail. Before the shearing is comple...

Cellulose nanocrystals (CNCs) are ubiquitous in some of nature’s toughest biological nanocomposites such as wood and bacterial cell walls [1] and exhibit outstanding mechanical properties rivaling those of synthetic materials such as Kevlar [2]. Although the impressive elastic properties of CNCs and mechanical properties of macroscale cellulosic materials have been well characterized, a molecul...

Removing the green husk and drying walnut are one of the most important steps in post-harvest processing of walnut. Therefore, it is need to precisely identify a walnut’s shell. Physical and mechanical properties of shell in the four Persian walnut cultivars/genotypes were studied. This study investigated the mechanical properties of a walnut’s husk and calculated the required energy in the pro...