Abstract. Agustina S, Wiryawan KG, Suharti Meryandini A. 2021. The enrichment process and morphological identification of anaerobic fungi isolated from buffalo rumen. Biodiversitas 23: 469-477. Anaerobic are one the microbes that have an important role in rumen fiber degradation because they can produce cellulase enzymes penetrate feed particles. Nevertheless, few studies were performed to test...

A fed-batch, anaerobic culture system was developed to assess the behavior of Escherichia coli O157:H7 in a rumen-like environment. Fermentation medium consisted of either 50% (vol/vol) raw or sterile rumen fluid and 50% phosphate buffer. Additional rumen fluid was added twice per day, and samples were removed three times per day to simulate the exiting of digesta and microbes from the rumen en...

A mixture of L-(U-14C) amino acids was added to cultures of 11 strains of rumen anaerobic fungi belonging to Neocallimastix frontalis, Neocallimastix joyonii, Sphaeromonas communis and Piromonas communis. Fungi were grown in a complex medium with glucose for 4 days. The radioactivity was found in cellular protein (27.7-65.3% of the total radioactivity recovered), lactate (16.9-41.8%), volatile ...

Anaerobic rumen microorganisms mainly bacteria, protozoa and fungi degrade ligno-cellulosic feeds consumed by the ruminants. The ruminants in developing countries are predominantly maintained on low grade roughage and grazing on degraded range land resulting in their poor nutrient utilization and productivity. Hence, manipulation of rumen fermentation was tried during last two decades to optimi...

This study investigated the effects of disodium fumarate (DF) on methane emission, ruminal fermentation and microbial abundance in goats under different forage (F) : concentrate (C) ratios and fed according to maintenance requirements. Four ruminally fistulated, castrated male goats were used in a 4 × 4 Latin square design with a 2 × 2 factorial arrangement of treatments and the main factors be...

An extensive in vitro study was conducted to delineate the influence of levels and source of sulfur besides rumen fungi habitat on the degradation of pearl millet stover dry matter and fibrous constituents. The results revealed that degradability of dry matter, organic matter, acid detergent fibre, neutral detergent fibre and cellulose from pearl millet stover was maximized (P<0.05), irrespecti...

Establishing the phylogeny of fungi and protists often has proved difficult owing to the simple morphologies and convergent characters in these organisms. We used DNA sequences of nuclear small-subunit ribosomal RNA genes to determine phylogenetic relationships among three major classes of organisms considered to be fungi--Basidiomycetes, Ascomycetes and Chytridiomycetes--and to assess the taxo...

the aim of this study was to determine effects of different fatty acid profiles on rumen digestion coefficients, some of rumen metabolism parameters and rumen microbial populations. different oils were used as source of fatty acids such as, oleic, linoleic, linolenic, dha and epa. palmitic acid supplemented from a commercial product (bergafat t-300) containing high palmitic acid percentage. res...