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The present study is designed to evaluate the application of a simple method for rapid identification of Malassezia furfur among other Malassezia spp. based on production of a brownish-red pigment and its diffusion into the medium containing L-tryptophan as a sigle source of nitrogen. 91 strains of Malassezia (20% M. furfur, 2% M. sympodialis, 5% M. obtosa and 73% M. globosa) isolated from ski...
Alkaline phosphatase (ortho phosphate, monoester hydrolase phosphoinositide E. C. 3.1.3.1) is a non-specific metalloproteinase enzyme that is located inside periplasmic space of bacteria. This enzyme is used to measure freshwater sediment in genetic engineering for cleaning water. Isolation and molecular identification of the bacteria producing alkaline phosphatase and comparison of its product...
background: this study was performed to induce conversion of rh strain tachyzoites of toxoplasma gondii to bradyzoites by ph changing of the culture medium. methods: hela cell monolayers were infected at a 1:1 tachyzoite to cell ratio. four hours after infection, the culture medium was removed and replaced with culture medium and 5% fcs, adjusted to ph 8 with naoh. the culture was maintained ...
conclusions in this study, snpcr was specific and more sensitive than the chromogenic medium chromagar™ candida for the detection of candida spp. insofar as it failed to identify only a few isolates. materials and methods we used snpcr with universal and species-specific primers to detect candida species in the culture of clinical isolates. by using universal primers, we carried out the amplifi...
Introduction: Streptomyces species are mycelial, aerobic gram-positive bacteria that are isolated from soil and produce a diverse range of antibiotics. Streptomyces griseus produces the antibiotic, streptomycin and forms spores even in a liquid culture. The gene cluster for the production of Streptomycin antibiotic contains strR gene that encodes StrR, a pathway-specific regulator. Then, this p...
The present article investigates the elasto-thermodiffusive interactions in a transversely isotropic elastic medium in the context of thermoelasticity with one relaxation time parameter and two relation time parameters. The resulting non-dimensional coupled equations are applied to a specific problem of a half-space in which the surface is free of tractions and is subjected to time-dependent th...
Introduction: Bacteriophages are viruses that infect and destroy prokaryote cells, specifically the bacteria. They act too selective, so as each bacteriophage affects only on specific type of bacteria. Due to their specific features, bacteriophages can be used as an appropriate substitute for antibiotics in infectious diseases treatment. Therefore, this study aimed to isolate E. coli-specific b...
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