Real-time reverse transcription quantitative PCR (RT-qPCR) is an important tool for gene expression analysis. Suitable reference genes are the basis of accurate and reliable RT-qPCR results. Litchi (Litchi chinensis Sonn.) a commercially tropical subtropical fruit, but rapid pericarp browning substantial negative impact on its commercial use. Reference validation could help in screening involve...

Yu A.-N., Tang L.-P. (2016): Kinetics of non-enzymatic browning reaction from the l-ascorbic acid/l-cysteine model system. Czech J. Food Sci., 34: 503–510. The kinetics of the non-enzymatic browning reaction from l-ascorbic acid/l-cysteine model systems was investigated at 125–155°C for 10–120 min by measuring the loss of reactants and monitoring the brown colour development. The result showed ...

About 170 species of Commelina are known from tropical and subtropical regions of the world. Nepal contributes 6 species of Commelina to the world flora. In the present work, taxonomy of all the six species of Commelina reported from Nepal (C. benghalensis, C. caroliniana, C. diffusa, C. maculata, C. paludosa and C. suffruticosa) was studied. Voucher specimens were collected from Central and Ea...

BACKGROUND AND AIMS This study investigates 47 taxonomically related species (Gentianales), all native to a tropical montane forest in southern Ecuador, in terms of nectar chemistry and nectar volumes in relation to pollination biology. METHODS Nectar volumes of covered (24-h production) and uncovered (standing crop) flowers were measured in the natural habitat. Sucrose, fructose and glucose ...

The spatial distribution of disturbances in Andean tropical forests and protected areas has commonly been calculated using bi or tri-temporal analysis because of persistent cloud cover and complex topography. Long-term trends of vegetative decline (browning) or improvement (greening) have thus not been evaluated despite their importance for assessing conservation strategy implementation in regi...

Understory herbs are an essential part of tropical rain forests, but little is known about factors limiting their reproduction. Many of these herbs are clonal, patchily distributed, and produce large floral displays of nectar-rich 1-d flowers to attract hummingbird pollinators that may transport pollen over long distances. The aim of this study was to investigate the effects of clonality, cross...

Fresh-cut leafy vegetable has an image as a healthy, nutritious, and delicious product. However, the product still faces challenge of quality retention short shelf life, especially in tropical climate regions. Enzymatic browning fresh-cut vegetables is considered one most important attributes limiting life The hurdle approach using commercial vacuum precooling combination with modified atmosphe...

Background: The enzyme tyrosinase is a copper-containing polyphenol oxidase, which is involved in melanogenesis. It catalyzes the conversion of tyrosine to melanin pigments in mammals. Tyrosinase plays an important role in enzymatic browning, which causes discoloration and loss economic value of many plant food products. This enzyme is widely distributed in fungi, higher plants and animals. Ob...

Peel browning disorder has an enormous impact on the exterior quality of 'Huangguan' pear whereas the underlying mechanism is still unclear. Although different methods have been applied for inhibiting the peel browning of 'Huangguan' pear, there are numerous issues associated with these approaches, such as time cost, efficacy, safety and stability. In this study, to develop a rapid, efficient a...