dispersive liquid-liquid microextraction method (dllme) combined with high-performance liquid chromatography-ultraviolet detection (hplc-uv) was used to determine thiamine (b1), nicotinamide (b3) and pyridoxine (b6) in sour cherry juice. this method was rapid, simple and sensitive. separation was accomplished using a c18 column. the optimum chromatographic conditions were found to be: mobile ph...

Dispersive liquid-liquid microextraction method (DLLME) combined with high-performance liquid chromatography-ultraviolet detection (HPLC-UV) was used to determine thiamine (B1), nicotinamide (B3) and pyridoxine (B6) in sour cherry juice. This method was rapid, simple and sensitive. Separation was accomplished using a C18 column. The optimum chromatographic conditions were found to be: mobile ph...

Dispersive liquid-liquid microextraction method (DLLME) combined with high-performance liquid chromatography-ultraviolet detection (HPLC-UV) was used to determine thiamine (B1), nicotinamide (B3) and pyridoxine (B6) in sour cherry juice. This method was rapid, simple and sensitive. Separation was accomplished using a C18 column. The optimum chromatographic conditions were found to be: mobile ph...

Immature cotyledons of open-pollinated fruits from three sour cherry cultivars (Prunus cerasus L.) were excised and cultured on Murashige and Skoog medium supplemented with various combinations of auxin and cytokinin to induce somatic embryogenesis. Somatic embryogenesis occurred principally when using the combinations of 2,4-dichlorophenoxyacetic acid plus kinetin. Using a-naphthaleneacetic ac...

In the last years many studies on anthocyanins, the flavonoids responsible for the orange, red and blue colors in different plant organs, have revealed their antioxidant activity and a possible use as chemotherapeutics. The finding that tart cherries (Prunus cerasus L.) contain a great amount of anthocyanins, constituents that possess strong antioxidant and anti-inflammatory activities, have at...

Thermotherapy and chemotherapy in vitro were applied to eliminate Apple chlorotic leaf spot virus (ACLSV) and Prunus necrotic ring spot virus (PNRSV) from myrobalan (Prunus cerasifera var. divaricata Borgh.), PNRSV from ‘Empress’ plum (Prunus domestica L.) and Prune dwarf virus (PDV) from ‘Early Rivers’ sweet cherry (Cerasus avium (L.) Moench.) plants. ELISA assays conducted one year after ther...

The objective of this study is to investigate the influence of cultivar and industrial processing on total polyphenols, anthocyanins, hydroxycinnamic acids and antioxidant activity in concentrated sour cherry (Prunus cerasus L., cvs. Marasca and Oblačinska) juices. Samples were collected during four processing steps: from fresh fruit prior to processing, then from pressed, filtered and concentr...