Background Infertility affects around 15% of couples, and male factors account for 50%. Cryptorchidism is one of the most common causes for azoospermia. Generation of functional spermatids from azoospermia patients is of unusual significance for treating male infertility. It has been recently reported by peers and us that human spermatogonial stem cells (SSCs) can be clearly identified and cult...

poultry spermatogonial stem cells (sscs) have the potential to serve as a model for studying the basic biology of ssc and they can also be used for biotechnological purposes. however, the small number of sscs and the presence of the testicular somatic cells with sscs have limited their applications. therefore, this study was undertaken for the first time to investigate the effect of a serum-fre...

carcinogenesis is a multistep process characterized by the gradual accumulation of genetic changes that ultimately lead to cancer. these genetic mutations can impart limitless replicative potential to the cancer cells making them immortal. telomeres are repeat nucleotide sequence ttaggg that are present at the end of chromosomes. its functions are to protect the chromosomal ends and to ensure t...

DNA extraction is usually the first step to perform molecular studies. This process can be nonviable due genomic (gDNA) commercial kits prices. Furthermore, available protocols generally have high specificity, limiting their use specific sources of biological material. In order reduce costs, optimize time and laboratory logistics, besides demonstrate a versatile protocol, present study worked o...

Somatic cell nuclear transfer (SCNT), the technique commonly known as cloning, permits transformation of a somatic cell into an undifferentiated zygote with the potential to develop into a newborn animal (i.e., a clone). In somatic cells, chromatin is programmed to repress most genes and express some, depending on the tissue. It is evident that the enucleated oocyte provides the environment in ...

abstract: mastitis is an inflammatory reaction of the mammary tissue in response to the introduction and multiplication of pathogens and is characterized by increasing somatic cell count (scc) in milk. the main objective of present study is to assess the probable associations between somatic cell (sc) levels and calcium and phosphorus and ratio of ca/p in raw milk. milk samples were collected f...

The ectopic expression of the key transcription factors Oct4, Sox2, c-Myc, and Klf-4 have been shown to reprogram somatic cells to a pluripotent state. In turn these induced pluripotent stem (iPS) cells, like embryonic stem (ES) cells, have been shown to be able to reprogram somatic cells by cell fusion. In this study we compare the differences and similarities between ES and iPS cells measured...

milk samples were collected from quarters of 35 cows with subclinical mastitis (california mastitis test [cmt] positive and somatic cell counts [scc] >500,000 cells/ml in individual quarter foremilk), as well as from 37 healthy controls. compared to the levels observed in milk from healthy quarters, milk from quarters with subclinical mastitis showed elevated sodium (91.97 vs 52.93 mg/dl), chlo...

BACKGROUND: Microbiological and chemical qualities ofmilk are among the most important issues in public health.OBJECTIVES:Although there are a few published studies whichhave been conducted in south east of Iran this study wasperformed to investigate quality of raw milk delivered to a dairyindustry company Kerman Province. METHODS:Atotal of 109raw milk samples were collected at the time of deli...