Background: The beneficial effect of supplementing culture medium with melatonin has been reported during in vitro embryo development of species such as mouse, bovine and porcine. However, the effect of melatonin on the mouse somatic cell nuclear transfer remained unknown. Materials and Methods: In this study, we assessed the effects of various concentrations of melatonin (10-6 to 10-12 M) on t...

This experiment was aimed to study the association between the DGAT1 K232A polymorphism and milk production traits and somatic cell score (SCS) in Iranian Holstein dairy cows. The records of 408 animals from five dairy herds were randomly identified and then genomic DNA was extracted from blood using the modified-salting method described by Miller. RFLP-PCR was performed to obtain all the polym...

Background Despite successful cloning of many mammalian species, the cloning efficiency is extremely low compared to that of IVF raising the possibility of the existence of epigenetic barrier preventing successful cloning. MaterialsAndMethods Using comparative transcriptome analysis comparing transcriptomes of IVF and SCNT embryos and that of donor cells, we identified epigenetic barrier and fi...

Somatic cell nuclear transfer (SCNT or cloning) returns a differentiated cell to a totipotent status; a process termed nuclear reprogramming. Reproductive cloning has potential applications in both agriculture and biomedicine, but is limited by low efficiency. To understand the deficiencies of nuclear reprogramming, our research has focused on both candidate genes and global gene expression pat...

the objective of this study was to estimate variance components, genetic and phenotypic trends for somatic cell score. dataset that collected by the animal breeding center of iran was used in this study. data file included 850,729 test day records of somatic cell counts (scc)  on 32,955 cows from lactations one to four, in 472 herds, gathered from years 2002 to 2013 in iran. a test day repeatab...

Association between Single Nucleotide Ppolymorphism (SNP) in the Intronic Region of (C2239T) B4-Defensin Gene with Milk Production Traits and Somatic Cell Count Holstein Cattle