Free-living diazotrophs are diverse and ubiquitous in soil, contributing the nitrogen pool in natural ecosystems. The isolation of nitrogen-fixing microorganisms has relied on semisolid nitrogen-free medium enrichment, followed by multiple subculturing steps. These procedures limit the diversity of recovered isolates. In the current study, we investigated three different isolation strategies fo...

the purpose of this study was to examine the effectiveness of nanosilver colloid as a new antimicrobial agent against erwinia in tissue culture of sugar beet for the recovery of the contaminated doubled haploid plantlets. the nanosilver colloidal solution of 4000 ppm was diluted in different concentrations (0, 50, 75, 100 and 125 ppm) and was added to the solid ms medium. in a factorial experim...