An attempt was made to purify Clostridium perfringens Beta toxin. Crude toxin prepared by ammonium sulphate precipitation of culture supernatants was purified by chromatography on Sephadex G50, Sephadex G100 and DEAE cellulose. This material, although highly purified was not homogeneous on polyacrylamide gel electrophoresis. It had a toxicity of 800 000 mouse MLDs/mg N, a typical protein absorp...

CoA-transferase (succinyl-CoA-3-oxo acid CoA-transferase, EC 2.8.3.5) isolated from sheep kidney was purified to homogeneity. The purified enzyme has a specific activity of approx. 200 units/mg. A mol.wt. of 110000 was obtained by gel filtration on Sephadex G-200, and a lower mol.wt. of 102000 was determined by analytical ultracentrifugation. A sedimentation coefficient of 5.6S was also determi...

Proteoglycans extracted from separated specimens of healthy human gingival epithelium and from connective tissue have been purified. The epithelial proteoglycans fractionated as a single included peak on Sepharose 4B-CL and contained heparan sulphate and dermatan sulphate glycosaminoglycans. The connective-tissue proteoglycans separated into three major populations on Sepharose 4B-CL, one of wh...

1. Strontium absorption was studied in vivo with loops of ileum in rachitic chicks and found to be increased by vitamin D(3), bile salts and sodium lauryl sulphate. 2. Bile salts and sodium lauryl sulphate rendered strontium soluble in butanol-benzene (1:1, v/v). 3. Bile was not concerned in the absorption of iron in rats from its water-soluble form, ferrous sulphate. 4. Ligation of the bile du...

kevlar is one of the fbers which is used in heavy industrial productions such as, shipping, military and weaving for reinforcement of composites. initial polymer of kevlar or poly(para-phenylene terephthalamide) is an oriented liquid crystal polymer. existence of aromatic group in the main chain leads to formation of rigid-rod molecules in the polymer, high strength and high modulus in it is fb...

The subunit of the Vicia graminea lectin with blood-group-N specificity was examined by sodium dodecyl sulphate/polyacrylamide-gel electrophoresis and gel filtration in 6M-guanidinium chloride, and its molecular weights was found to be 25 000. The unique N-terminal sequence fof the first nine residues of the lectin confirmed that Vicia lectin consists of four identical chains non-covalently lin...

The molecular weight and hydrodynamic properties of a polypeptide isolated from the lamellar system of Antirrhinum chloroplasts were determined in sodium dodecyl sulphate solution by measurement of sedimentation velocity, diffusion and effective partial specific volume. The polypeptide fraction exhibits a molecular weight of 25000 which agrees with the apparent molecular weight found by polyacr...

in study, spherical silver nanoparticles (snps) were synthesized by chemical reduction method from a metal precursor silver nitrate in presence of an anionic surfactant and strong reducing agent. in this experimental work, snps are synthesized in presence of different concentration of stabilizing agent and effect of stabilizing agent on size distribution of snps have been observed. further anti...

In defense of: • Glycerine (Page 4) • Cetyl Stearyl Alcohol (Page 5) • Animal Products (Beeswax, Lactic acid & Lanolin in one product each) (Page 5) • Para-aminobenzoic Acid (PABA) (Page 5) • Parabens (Page 6) o Hazardous and Useless Alternatives Exposed (Page 8) Silver Chloride (Page 8) Grapefruit Seed Extract [Adulterated with Benzethonium Chloride & Triclosan] (Page 8) In defense of: • Sodiu...

The present work was conducted on the effect of surfactant on the dissolution rate of ibuprofen tablets. The cationic (Cetyl Trimethyl bromide) and anionic (Sodium dodecyl sulphate) surfactant present in the dissolution media have remarkable effect on the dissolution rate of Ibuprofen. Maximum dissolution was obtained in the presence of anionic surfactant (Sodium dodecyl sulphate) at 0.5% conce...