Glomerular visceral epithelial cells are endowed with a sialic acid-rich surface coat (the "glomerular epithelial polyanion"), which in rat tissue contains the sialoprotein podocalyxin. We have identified a major membrane sialoprotein in human glomeruli that is similar to rat podocalyxin in its sialic acid-dependent binding of wheat germ agglutinin and in its localization on the surface of glom...

The gating component associated with the voltage-sensitive Na+ channel from electroplax membranes of Electrophorus electricus has been purified by using toxin gamma from the venom of the scorpion Tityus serrulatus serrulatus. The toxin-binding site was efficiently solubilized with Lubrol PX, resulting in an extract of high initial specific activity. Purification was achieved by adsorption of th...

The role of surface proteins in Vibrio cholerae attachment to chitin particles in vitro was studied. Treatment of V. cholerae O1 ATCC 14034 and ATCC 14035 with pronase E reduced the attachment of bacteria to chitin particles by 57 to 77%. A statistically significant reduction was also observed when the attachment to chitin was evaluated in the presence of homologous Sarkosyl-insoluble membrane ...

T-antigens from simian virus 40 (SV 40)-transformed and lytically infected cells have been isolated by immunoprecipitation and their molecular weights estimated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. T-antigen from SV40-transformed mouse and hamster cells has an apparent molecular weight of 94,000 whereas that from several lines of SV40-infected monkey cells is 84,000. In...

Vesicular stomatitis virus messenger RNA has been transcribed in vitro from the viral genome by the virion-associated RNA polymerase in quantities suitable for translation. Wheat germ cell-free extracts programmed with the isolated in vitro 12-18S RNA fraction synthesize polypeptides similar to the viral N, NS, and M proteins, as judged by sodium dodecyl sulfate-polyacrylamide gel electrophores...

In order to explore how molecules are linked to the membrane surface in larval Taenia solium, whole cysticerci were incubated in the presence of phospholipase C from Clostridium perfringens (PLC). Released material was collected and analyzed in polyacrylamide gels with sodium dodecyl sulfate. Two major bands with apparent molecular weights of 180 and 43 kDa were observed. Western blot of releas...

Chicken globin mRNA has been purified and partially characterized. Globin-specific sequences are found primarily as 9 S RNA, but also are found with ribosomal RNA, preferentially the 28 S moiety. The chicken globin mRNA preparation has been translated in the wheat germ and Krebs ascites cell-free systems. The products have been identified by sodium dodecyl sulfate-gel electrophoresis as the alp...

fractional flow analysis confirms the advantages of surfactant alternative gas injection (sag) in enhanced oil recovery, but an adsorption phenomenon that has been affected by several factors, weakens the effectiveness of sag injection. in this study, the effects of sacrificial agent, gas phase, and surfactant concentration on adsorption density on silica mineral were investigated by static and...

Surfactants are chemicals that adsorb to the interface between a solution and another phase (gases or solids) self-assemble molecular clusters known as micelles in (water oil phase). Measurements of surface tension sodium dodecyl sulfate (SDS) distilled water at 298.15K reported. The increase concentration then decreased 298.15K. Critical micelle (CMC) with decrease tension. graphs versus were ...

Polysomes prepared from cultured Chinese hamster lung cells direct the synthesis of procollagen (Y chains in an heterologous cell-free system containing the postribosomal supernatant fraction prepared from wheat germ. Total protein synthesis requires both subcellular components and an exogenous energy source, and is inhibited by the antibiotics puromycin and am-in tricarboxylic acid. The ratio ...