Appropriate methodologies for the determination of drug penetration in diseased skin have not yet been established. The aim of this study was to determine the cutaneous penetration of a metronidazole cream formulation in atopic dermatitis, employing dermal microdialysis and tape strip sampling techniques. Non-invasive measuring methods were used for the quantification of the severity of the der...

The aim of this study was twofold, that is the generation of a reliable model for skin barrier disruption and repair and to evaluate recovery of damaged skin after application of vernix caseosa (VC). VC was selected as its wound healing properties were suggested previously, but never clearly demonstrated. Five different levels of barrier disruption in mice, accomplished by tape-stripping, were ...

Tape strips have been used widely in dermatology research as a minimally invasive method to sample the epidermis, avoiding need for skin biopsies. Most has focused on epidermal pathology, such atopic eczema, but there is increasing into use of tape other dermatoses, cancer, and microbiome. This review summarizes technique stripping, discusses which dermatoses studied by stripping alternative sa...

BACKGROUND Transient receptor potential vanilloid type 1 (TRPV1) is a cation channel activated by diverse obnoxious stimuli like capsaicin, low pH or heat. Recently, it was revealed that TRPV1 might be deeply associated with skin permeability barrier function, suggesting that modulation of TRPV1 might be beneficial for the skin disorders with barrier damages. OBJECTIVE We aimed to investigate...

BACKGROUND Percutaneous penetration of urea in vivo in man has been documented. If urea can penetrate the skin, it may also move laterally. Lateral spreading of topical substances leads to unpredictable penetration dynamics and increased skin surface area exposure. METHODS The ability of urea, a low molecular-weight hydrophilic model, to penetrate the stratum corneum (SC) and spread outside t...

The influence of the ligand/metal ion concentration ratio on the shape, peak current and peak potential of curves obtained by anodic stripping voltammetry (a.s.v.) at the hanging mercury drop electrode is described, particularly with respect to the use of a.s.v. for speciation of metal ions at very low concentrations as is often found in natural waters. The lead(II)/triethylenetetramine system ...

BACKGROUND/PURPOSE To develop modification of stripping method allowing high-resolution fluorescent visualization of corneocytes of human skin in vitro. To validate the method, the measured corneocyte areas on skin flakes are collected from individuals of different ages. MATERIALS AND METHODS Two complimentary fluorescent dyes were used sequentially. First the adhesive layer of the stripping ...

The effectiveness of three adhesive tapes, 3M’s No. 810, No. 845 and No. 850, in stripping stratum comeum from hairless mouse skin. was compared by measuring transdermal water loss (TEWL) and distribution profiles of a marker, minoxidil, from a solution formulation. While No. 850 tape failed to increase TEWL perceptively even after 24 strippings, both No. 810 and 845 tapes effectively removed t...

It is well known that lead and cadmium can be determined with good sensitivity using anodic stripping voltammetry (ASV) and a mercury ®lm electrode. However, the mercury ®lm formation is poorly reproducible and cannot fully be removed electrochemically. We have evaluated procedures to improve the reproducibility and sensitivity of the ASV method and found that in the presence of thiocyanate the...