introduction: the purpose of this study is to evaluated the effect of low power laser on the survival of a skin flap on the dorsum of the rats. materials and methods: fifty male rats were divided into five groups. the surgery was done under general anesthesia where a full thickness skin flap (including panniculus carnosus) from the dorsum of the rats were randomly dissected. the flap size was 2...

OBJECTIVE To study the application value of artificial skin substitute compositely constructed by adipose-derived stem cells and fibrin gel in skin defect. MATERIALS AND METHODS Adipose-derived stem cells (ADSCs) were obtained from healthy pure green fluorescent protein (GFP) transgenic mice and were proved to have multiple differentiation potentials. They compositely constructed artificial s...

PURPOSE To investigate the effects of pharmacological delay with insulin-like growth factor-1 (IGF-1) on skin flap survival. METHODS Thirty Sprague-Dawley rats were submitted to dorsal skin flap (3x9 cm). Seven days before the surgery, the animals were subdivided into three groups of 10 rats. In group 1 (controls), no injection was done. Seven days before the elevation, saline had been inject...

Adenoviral transduction of the VEGF gene in an oversized skin flap increases flap survival and perfusion. In this study, we investigated the potential of magnetofection of magnetic lipospheres containing VEGF(165)-cDNA on survival and perfusion of ischemic skin flaps and evaluated the method with respect to the significance of applied magnetic field and ultrasound. We prepared perfluoropropane-...

closure of donor site of the flap has special problems. reduction of this site will decrease the morbidity of operation. in this study, we present our experience in donor site size reduction. methods between 2006 and 2008, 15 patients with skin and soft tissue defects underwent operation. in all patients, coverage of defect was performed with various flaps. substratum horizontal mat-tress sutur...

BACKGROUND The necrotic skin flap represents a great challenge in plastic and reconstructive surgery. In this study, we evaluated the effect of bioscaffolds, acellular amniotic membranes (AAMs), and bone marrow-derived mesenchymal stem cells (BM-MSCs) on random skin flap (RSF) survival in rats by applying a cell-free extracellular matrix scaffold as a supportive component for the growth and pro...

OBJECTIVE To determine the efficacy of topical anti-ischemic drug therapy in the salvage of failing, random-pattern skin flaps. DESIGN Prospective, randomized, placebo-controlled, therapeutic trial. SETTING Academic medical center. SUBJECTS Sixty-one adult male Sprague-Dawley rats. INTERVENTION Each experimental rat underwent a caudally based random-pattern skin flap using the modified ...

Skin flaps are frequently performed for diabetic patients in spite of countless detrimental effects of diabetes on flap survival, most of which may result from a defective response of the tissues to low oxygen tension. In this study, the authors explored the feasibility of applying human adipose-derived stem cells (ASCs) to increase the viability of random-patterned skin flaps in streptozotocin...

Accurate and early prediction of tissue viability is the most significant determinant of tissue flap survival in reconstructive surgery. Perturbation in tissue water content (TWC) is a generic component of the tissue response to such surgeries, and, therefore, may be an important diagnostic target for assessing the extent of flap viability in vivo. We have previously shown that reflective terah...