Abstract Background The role of viruses as a cause breast cancer (BC) has been significantly investigated in recent years. Human papillomavirus (HPV) detected invasive carcinomas, while most studies have only focused on the detection viral DNA, we aimed to examine prevalence and genotypes HPV among Iranian BC patients. We also examined presence herpes simplex-1 (HSV-1), simplex-2 (HSV-2), varic...

Canine infectious respiratory disease complex (CIRDC) viruses have been detected in dogs with respiratory illness. Canine influenza virus (CIV), canine parainfluenza virus (CPIV), canine distemper virus (CDV), canine respiratory coronavirus (CRCoV), canine adenovirus type 2 (CAdV-2) and canine herpesvirus 1 (CaHV-1), are all associated with the CIRDC. To allow diagnosis, two conventional multip...

Despite wide application to beach water monitoring and microbial source identification, results produced by quantitative PCR (qPCR) methods are subject to bias introduced by reliance on quantitative standards. Digital PCR technology provides direct, standards-free quantification and may potentially alleviate or greatly reduce other qPCR limitations such as difficulty in multiplexing and suscept...

Orolabial and genital herpes are common conditions caused by simplex virus (HSV) that affect many individuals. Although skin findings may mimic other conditions, an HSV infection still be diagnosed clinically. We present a case of polymerase chain reaction (PCR) negative orolabial in young male with high-risk social history. testing was negative, oral clinically the patient adequately treated I...

The feasibility of detecting herpesvirus DNA in paraffin sections of routinely fixed and processed human necropsy brains by use of the polymerase chain reaction (PCR) was assessed. A 110 bp segment of the thymidine kinase gene of herpes simplex virus type 1 (HSV1) could readily be amplified in sections from the brains of six patients with acute HSV1 encephalitis but not from those of six patien...

A 28 years old female presented with headache, fever, altered sensorium and right side weakness for one week. She was febrile and drowsy with right sided hemiplegia and papilledema. Tuberculous or bacterial meningitis, tuberculoma and abscess were at the top of the diagnosis list followed by Herpes simplex meningo-encephalitis (HSE). MRI showed abnormal signal intensity of left temporal lobe wi...

We report the clinical case of a genital outbreak with both Herpes Simplex Type 1 (HSV-1) and Herpes Simplex Type 2 (HSV-2) during pregnancy. Herpes was presumptively identified by clinical presentation of lesion and Tzanck smear while serotypes were identified by cell culture and polymerase chain reaction (PCR). This case report highlights the need for increased surveillance of both serotypes ...

Herpes simplex virus (HSV) latency in sensory ganglion neurons is well documented, but the existence of extraneuronal corneal latency is less well defined. To investigate the possibility of extraneuronal latency during ocular HSV infection, corneal specimens from 18 patients with quiescent herpes simplex keratitis (HSK) were obtained at the time of keratoplasty. Polymerase chain reaction (PCR) ...

Asymptomatic genital shedding of herpes simplex virus type 2 (HSV-2) DNA was evidenced by real-time PCR in 25 (13.2%) of 188 cervicovaginal lavage samples and in only 13 (6.9%) paired cervicovaginal samples from 188 HSV-2-seropositive, nonpregnant childbearing-aged human immunodeficiency virus-seronegative women living in Gabon. These observations demonstrate that cervicovaginal washing is more...